Mapping Data

Experiment

• Experiment
  HYBRID
• Chromosome
  8
• Reference
  J:9626 Wang LM, et al., Chromosome assignment of mouse insulin, colony stimulating factor 1, and low-density lipoprotein receptors. Genomics. 1988 Aug;3(2):172-6
• ID
  MGI:38210

Genes

Gene
Insr

Notes

• Experiment
  Somatic cell hybrids were from fusion of Chinese hamster and mouse cells. EcoRI cleaved DNA from the hybrids was probed for mouse Insr using human cDNA probe phINSR 14-1. The assignment of Insr to Chromosome 8 was confirmed by the analysis of 4 rat x mouse microcell hybrids. Microcell hybrid F(8.14)Q carried a mouse Robertsonian chromosome composed of Chromosomes 8 and 14 and was positive for Insr. Hybrid F(8)D contained only Chromosome 8, and was positive for Insr. Hybrid FB(8)D and F(11)G, which lackedChromosome 8, also lacked the Insr gene.

HYBRID

• Concordance
  chromosomes

HYBRID Data

Chromosome	+/+	+/-	-/+	-/-
1	5	3	3	3
2	8	3	0	3
3	7	2	1	4
4	4	0	4	6
5	4	1	4	5
6	7	1	1	5
7	8	4	0	2
8	7	0	1	6
9	5	1	3	5
10	4	2	4	4
11	0	0	8	6
12	8	3	0	3
13	6	3	2	3
14	2	2	6	4
15	8	5	0	1
16	7	3	1	3
17	8	2	0	4
18	4	1	4	5
19	7	0	1	6
X	8	6	0	0