Mapping Data

Experiment

• Experiment
  TEXT-Genetic Cross
• Chromosome
  3
• Reference
  J:136642 Matera I, et al., A sensitized mutagenesis screen identifies Gli3 as a modifier of Sox10 neurocristopathy. Hum Mol Genet. 2008 Jul 15;17(14):2118-31
• ID
  MGI:3822500

Genes

Gene
Mos3
D3Mit178
D3Mit65

Notes

• Experiment
  Mapping analyis of over 200 pedigrees identified three Sox10 modifiers , named modifier of Sox10 (Mos1, Mos2 and Mos3). Linkage indicated a locations on mouse Chromosomes 13, 4 and 3, respectively, For mapping of Gli3 ^Mos1, nine mice were
  used for an initial genome scan (six affected Sox10LacZ/+; Mos1/+ mice and three
  Mos1/+ heterozygous carriers). Typing of 220 SNPs with alleles in
  C57BL/6J and BALB/cJ identified a 20 Mb region of proximal mouse Chr 13 where all
  nine animals had inherited the BALB/cJ allele from the affected founder animal,
  providing evidence for linkage to markers in this region including Gli3 wherein Mos1 was determined to be allelic.
  
  Mos2 and Mos3 were mapped to mouse Chromosome 4 and 3, respectively. With Mos2, the authors detected linkage to mouse Chromsome 4 by utilizing 91 SSLP
  markers to genotype eight affected animals. Subsequent genotyping of affected animals
  (N=31) in this region localized Mos2 to 35 Mb region of Chr 4 flanked by markers
  D4Mit9 (1/31 recombinant;P< 0.0001) and D4Mit203 (8/31 recombinant; P < 0.01). For
  Mos3, genotyping 408 SNPs in five affected animals showed linkage
  to two regions of the genome. Subsequent genotyping of SSLPs spanning those regions in
  additional affected animals (N=32) refinedthe linkage to a 20 Mb region of mouse Chromosome 3 flanked by markers D3Mit178 (2/32 recombinant; P < 0.001) and D3Mit65 (3/32 recombinant; P
  < 0.0001).