Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  6
• Reference
  J:142667 Shao H, et al., Genetic architecture of complex traits: large phenotypic effects and pervasive epistasis. Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19910-4
• ID
  MGI:3822817

Genes

Gene	Assay Type
Obrq1	visible phenotype
Obrq2	visible phenotype
Obrq3	visible phenotype
Obrq4	visible phenotype
D6Mit284	PCR amplified length variant
D6Mit15	PCR amplified length variant
D6Mit138	PCR amplified length variant
D6Mit159	PCR amplified length variant
D6Mit223	PCR amplified length variant
D6Mit274	PCR amplified length variant

Notes

• Experiment
  Chromosome substitution strains derived from A/J donor chromosomes on a C57BL/6J genetic background were used to map QTL associated with 90 phenotypes including blood, bone and metabolic traits. Intercrosses between (C57BL/6J-Chr 6^A/J/NaJ x C57BL/6J)F2and (C57BL/6J-Chr 10^A/J/NaJ x C57BL/6J)F2 mapped several obesity and liver triglyceride QTL on chromosomes 6 and 10. Animals were placed on a high fat diet at 5 weeks of age and weighed every 2 weeks for 100 days. Background strain C57BL/6J displays increased body weight and liver triglyceride levels compared to donor strain A/J.
  
  Two traits were explored in congenic strains containing nested series of segments derived from chromsomes 6 and 10; CSS-6 and -10 for diet-induced obesity and CSS-10 for levels of liver triglycerides.
  
  Obrq1 (obesity resistance QTL 1), Obrq2, Obrq3 and Obrq4 mapped to mouse Chromosome 6. The order of the QTL is centromere - Obrq2 - Obrq3 - Obrq4 - Obrq1 - telomere.
  
  Obrq2 was mapped in 6C2 (B6.A-(D6Mit138-D6Mit159)/6C2Na) and was linked with D6Mit159 within the congenic interval.
  Obrq3 was mapped in 6C3 (B6.A-(D6Mit138-D6Mit223)/6C3Na) and was linked with D6Mit223 within the congenic interval.
  Obrq4 was mapped in 6C4 (B6.A-(D6Mit138-D6Mit274)/6C4/Na and was linked D6Mit274 within the congenic interval.
  Obrq1 was mapped in 6C12 (B6.A-(D6Mit284-D6Mit15)/6C12Na) and was flanked within the congenic interval by D6Mit284 and D6Mit287 within the interval. [Fig.3, SI]
  
  QTL identified on the Chromosome 10:
  Obrq9 (obesity resistance QTL 9) was mapped in 10C1 (B6.A-rs29331213/10C1Na) linked with rs29331213 within the interval.
  Obrq10 was mapped in 10C2 (B6.A-(rs13480792-rs29331213)/10C2Na) and is linked with rs13480792 within the congenic interval.
  Obrq11 was mapped in 10C6 (B6.A-(D10Mit230-rs29331213)/10C6Na) flanked by markers D10Mit230 and D10Mit95 within the congenic interval.
  Obrq12 was mapped in 10C7 (B6.A-(rs13480651-rs29331213)/10C7Na) flanked by markers rs13480651 and D10Mit42 within the congenic interval.
  Ltgq1 was mapped in 10C9 (B6.A-(rs29359333-rs29375724)/10C9Na) linked with rs29359333.
  Ltgq2 (liver triglyceride QTL 1) was mapped in 10C1 (B6.A-rs29331213/10C1Na)and was also linked with rs29331213.
  The order of liver triglyceride and and obesity QTL is centromere - Ltgq1 -Obrq12 - Obrq11 - Obrq10 - Obrq9/Ltgq2 - telomere. [ Fig S2 and SI].