Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  9
• Reference
  J:142770 Stylianou IM, et al., Differences in DBA/1J and DBA/2J reveal lipid QTL genes. J Lipid Res. 2008 Nov;49(11):2402-13
• ID
  MGI:3828098

Genes

Gene	Assay Type
Tgq19	visible phenotype
Hdlq62	visible phenotype

Notes

• Experiment
  Linkage analysis was performed using a backcross derived from closely related inbred strains DBA/1J and DBA/2J. The genetic composition of these 2 inbred strains differs by just 5.6% at the SNP level. A total of 294 mice from (DBA/1J x DBA/2J)F1 x DBA/1J and (DBA/1J x DBA/2J)F1 x DBA/2J populations were screened for 6,949 polymorphic SNP markers. Backcross mice were placed on a high-fat diet at 8 weeks of age and phenotyped for plasma triglyceride and HDL concentrations at 8, 11 and 16 weeks of age. Parental strain DBA/2J displays significantly increased plasma triglyceride concentrations on a CHOW diet compared to parental strain DBA/1J. Male DBA/2J animals display significantly increased plasma triglyceride and HDL concentrations after an 8 week high fat diet compared to DBA/1J males.
  
  Tgq19 (triglyceride QTL 19) on mouse Chromosome 9 is linked to plasma triglycerides after 3 and 8 weeks of high fat diet. Peak association to 3-week triglycerides occurs at 18 cM (LOD=4.1) and peak association to 8-week triglycerides occurs at 6 cM (LOD=4.7). The peak marker is rs3023961 (4 Mb) [Table 2 and 3]. DBA/2J-derived alleles at Tgq19 confer increased plasma triglycerides. The QTL confidence interval spans 0 cM to 20 cM.
  
  Significant linkage to HDL concentrationafter 8 week high fat diet mapped to 1 cM with LOD=4.1. This locus is named Hdlq62 (HDL QTL 62).