Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  14
• Reference
  J:142770 Stylianou IM, et al., Differences in DBA/1J and DBA/2J reveal lipid QTL genes. J Lipid Res. 2008 Nov;49(11):2402-13
• ID
  MGI:3828431

Genes

Gene	Assay Type
Tgq25	visible phenotype
Tgq26	visible phenotype

Notes

• Experiment
  Linkage analysis was performed using a backcross derived from closely related inbred strains DBA/1J and DBA/2J. The genetic composition of these 2 inbred strains differs by just 5.6% at the SNP level. A total of 294 mice from (DBA/1J x DBA/2J)F1 x DBA/1Jand (DBA/1J x DBA/2J)F1 x DBA/2J populations were screened for 6,949 polymorphic SNP markers. Backcross mice were placed on a high-fat diet at 8 weeks of age and phenotyped for plasma triglyceride and HDL concentrations at 8, 11 and 16 weeks of age. Parental strain DBA/2J displays significantly increased plasma triglyceride concentrations on a CHOW diet compared to parental strain DBA/1J. Male DBA/2J animals display significantly increased plasma triglyceride and HDL concentrations after an 8 week high fat diet compared to DBA/1J males.
  
  Linkage to plasma triglyceride concentrations in males on a CHOW diet mapped to 48 cM on mouse Chromosome 14 at rs3686670 (LOD=4.4). This locus is named Tgq25 (triglyceride QTL 25). The QTL confidence interval spans 22 cM - 48 cM.
  
  A second locus named Tgq26 mapped to 25 cM at rs3090594 (57.7 Mb) and is linked to plasma triglycerides in males on a high fat diet for 8 weeks (LOD=4.6). The confidence interval for Tgq26 spans 7 cM - 36 cM. DBA/2J-derived alleles confer increased plasma triglycerides at Tgq25 and Tgq26.