Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  1
• Reference
  J:145043 Ligons DL, et al., A locus on chromosome 1 promotes susceptibility of experimental autoimmune myocarditis and lymphocyte cell death. Clin Immunol. 2009 Jan;130(1):74-82
• ID
  MGI:3836282

Genes

Gene	Assay Type
Eamcd1	resistance/susceptibility
D1Mit65	PCR amplified length variant
D1Mit334	PCR amplified length variant

Notes

• Experiment
  A previously mapped genetic locus on mouse Chromosome 1 exhibiting suggestive linkage to autoimmune myocarditis susceptibility was confirmed in this study using congenic line analysis. A 50 cM region (D1Mit65 to D1Mit334) containing Eamcd1 (experimental autoimmune myocarditis 1) derived from myocarditis susceptible strain A.SW-H2^s/J was isolated on a resistant B10.S-H2^s/J genetic background. This congenic line displays increased incidence of autoimmune myocarditis and is designated B10.A-(D1Mit65-D1Mit334). Experimental autoimmune myocarditis is characterized by the production of cardiac-specfic autoantibodies and diffuse mononuclear infiltratation after immunization with mouse cardiac myosin. Congenic animals also displayed decreased activation of caspase 3, 8 and 9 in CD4+ T cells after myosin antigen challenge. However, the severity of induced autoimmune myocarditis in B10.A-(D1Mit65-D1Mit334) congenic animals is lessened compared to that of susceptible donor strain A.SW.
  
  Icos (32 cM) and Il1r2 (19.5 cM) are two potential candidate genes located in the Eamcd1 QTL interval from 8.4 cM to 49.7 cM. Following myosin antigen challenge, Icos and Il1r2 mRNA expression is higher in the lymph nodes of B10.S-H2^s/J mice compared to A.SW-H2^s/J mice.