Mapping Data

Experiment

• Experiment
  TEXT-Congenic
• Chromosome
  1
• Reference
  J:145597 Hofstetter JR, et al., Characterization of the quantitative trait locus for haloperidol-induced catalepsy on distal mouse chromosome 1. Genes Brain Behav. 2008 Mar;7(2):214-23
• ID
  MGI:3838611

Genes

Gene	Assay Type
Hpic2	visible phenotype
D1Mit145	PCR amplified length variant
D1Mit17	PCR amplified length variant
D1Mit403	PCR amplified length variant
D1Mit273	PCR amplified length variant
D1Mit426	PCR amplified length variant

Notes

• Experiment
  A previously identified QTL for haloperidol-induced catalepsy, Hpic2 (haloperidol induced catalepsy 2), on distal mouse Chromosome 1 was confirmed using congenic line B6.D2-Mtv7a/TyJ, which carries DBA/2J-derived DNA from D1Mit145 (89 cM; 169.1 Mb) to D1Mit17 (106.3 cM; 191.3 Mb) on a C57BL/6J genetic background. B6.D2-Mtv7a/TyJ and DBA/2J donor strain mice are sensitive to haloperidol-induced catalepsy. Fifteen minutes after haloperidol injection the animals were placed in fixed rearing position. Catalepsy-sensitive animals maintained the rearing position for over 30 seconds. C57BL/6J animals do not display this catalepsy response after haloperidol administration.
  
  The Hpic2 locus was refined further using a series of interval specific congenic lines derived from B6.D2-Mtv7a/TyJ backcrossed to C57BL/6J and selecting for recombination across the congenic interval. This method localized Hpic2 to a 5.9 Mb interval between D1Mit403 (100 cM; 177.5 Mb) to D1Mit273 (102.5 cM; 183.4 Mb) with the highest association to haloperidol sensitivity at 101 cM at D1Mit426 (P=0.0001). This interval contains 53 known and predicted genes.
  
  A screen for candidate genes with non-conservative amino acid changes and whole brain and/or striatum mRNA expression differences betweenC57BL/6J and DBA/2J was performed. Ephx1(98.5 cM) is a possible candidate gene for Hpic2 as it contains a non-conservative SNP, displays differential mRNA expression between C57BL/6J and DBA/2J, and is a cis-eQTL for haloperidol sensitivity. Lin9 (182.5 Mb) is a possible candidate with non-conservative SNP variations between C57BL/6J and DBA/2J. Cabc1 (182.09 Mb) is also a potential candidate for Hpic2, with differentially expressed striatum mRNA and weak linkage as a cis-eQTL. Potential candidate genes identified solely by mRNA expression variations between C57BL/6J and DBA/2J whole brain and striatal mRNA include Tmem63a (182.87 Mb), Parp1 (98.6 cM; 182.49 Mb), Psen2 (101 cM; 182.15 Mb), Nvl (183.02 Mb) and Cnih4 (183.08 Mb).