Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  1
• Reference
  J:144089 Su Z, et al., Genetic basis of HDL variation in 129/SvImJ and C57BL/6J mice: importance of testing candidate genes in targeted mutant mice. J Lipid Res. 2009 Jan;50(1):116-25
• ID
  MGI:3839751

Genes

Gene	Assay Type
Hdlq15	visible phenotype

Notes

• Experiment
  Linkage analysis was performed on 528 animals from a (C57BL/6J x 129S1/SvImJ)F2 intercross to map QTL associated with plasma HDL levels on either a CHOW or atherogenic diet. F2 animals were fed a CHOW diet until 8 weeks of age and then placed on an atherogenic diet until 16 weeks of age. An array of 508 SNPs was used for genome scan. Plasma HDL is significantly higher in 129S1/SvImJ parental strain compared to C57BL/7J on CHOW and high fat diets, with a stronger effect observed in males compared to females. Several previously identified HDL QTL were detected in this study as well as several novel loci, some of which showed interactive effects.
  
  A previously identified QTL on mouse Chromosome 1 named Hdlq15 (HDL QTL 15) mapped to 173.4 Mb (93.3 cM) near rs31507136. The Hdlq15 95% confidence interval spans 170 Mb (87.9 cM) to 175 Mb (97 cM). 129S1/SvImJ-derived alleles at Hdlq15 confer increased plasma HDL on a CHOW (LOD=68.6) or atherogenic diet (LOD=30). Apoa2 (92.6 cM; 173.15 Mb) is believed to be the QTL gene responsible for Hdlq15. Interaction was observed between Hdlq15 and a locus on mouse Chromosome 7 named Hdlq58 (HDL QTL 58). Homozygosity for 129S1/SvImJ-derived alleles at Hdlq15 and homozygosity for C57BL/6J-derived alleles at Hdlq58 confer significantly increased plasma HDL levels in male and female animals on CHOW diet. Hdlq15 also interacted with Hdlq60 (HDL QTL 60) on chromosome X. Homozygosity for 129S1/SvImJ-derived alleles at Hdlq15 in conjunction with at least one C57BL/6J-derived alleleat Hdlq60 significantly increased plasma HDL levels in males and females on atherogenic diet.