Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  2
• Reference
  J:144817 Seidman MA, et al., PECAM-independent thioglycollate peritonitis is associated with a locus on murine chromosome 2. PLoS One. 2009;4(1):e4316
• ID
  MGI:3845113

Genes

Gene	Assay Type
Pitgp	visible phenotype
D2Mit7	PCR amplified length variant

Notes

• Experiment
  The Pecam1^tm1Mak knockout allele on an FVB/N genetic background results in impaired inflammatory response due to a blockade in leukocyte transendothelial migration. However, when Pecam1^tm1Mak is on a C57BL/6 genetic background inflammatory response and diapedesis are not impaired. Genetic modifiers responsible for this phenomenon were mapped by screening 150 polymorphic marker in a population of (B6.129P2-Pecam1^tm1Mak x FVB/N-Pecam1^tm1Mak)F2 intercross animals. Inflammatory response was measured after thioglycollate administration.
  
  Significant linkage to inflammatory response mapped to proximal mouse Chromosome 2 at 28 cM (38.2 Mb) near D2Mit7 (LOD=7.5). This locus is designated Pitgp (Pecam1-independent thioglycollate peritonitis). Additional analysis of Pitgp using medium-density mapping refined the QTL location to 35.8 Mb and increased the LOD score to 9.0. Four potential candidate genes are located near Pitgp: Ptgs1 (29 cM), Ptges (24 cM), Ptges2 and Hc (23.5 cM). C57BL/6-derived alleles atPitgp confer Pecam1-independent inflammatory response in a thioglycollate model of peritonitis, and appears to exhibit dominant inheritance. Authors speculate the gene responsible for Pitgp is a gain-of-function variant.