Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  18
• Reference
  J:145156 Vyskocilova M, et al., Polymorphism in hybrid male sterility in wild-derived Mus musculus musculus strains on proximal chromosome 17. Mamm Genome. 2009 Feb;20(2):83-91
• ID
  MGI:3846318

Genes

Gene	Assay Type
Mhysq5	visible phenotype
D18Mit149	PCR amplified length variant

Notes

• Experiment
  Crosses between C57BL/10J and M. m. musculus wild-derived phenotype selection lines STUF/Fore (Studenec fertile) and STUS/Fore (Studenec sterile) were used study genetic loci involved with hybrid male sterility. STUF males have increased mean testis weight, epididymis weight, and sperm count compared to STUS males. Thirty-three percent of males from a (STUS/Fore x STUF/Fore)F1 x C57BL10/J testcross are sterile and display decreased epididymis and testis size. Linkage analysis was performed using 103 polymorphic markers at an average resolution of 10 cM - 20 cM.
  
  Highly significant linkage to sperm count mapped to proximal mouse Chromosome 17 near D17CH07 and D17Mit43 with LRS=168.5 (LOD=36.63). This locus is also linked to testis weight (LRS=120.7) and epididymis weight (LRS=105.8) and is designate Mhysq1 (male hybrid sterility QTL 1). Mhysq1 accounts for 90% of the total additive genetic variance for sperm count, 83% of the variance for mean testis weight, and 77% of the variance for epididymis weight.STUS/Fore-derived alleles at Mhysq1 appear to confer low sperm count. Proximal mouse Chromosome 17 has previously been associated with hybrid male sterility as evidenced by multiple QTL occurring in this region: Hst1 (8.25 cM), Hst4 (14.2 cM), Hst6 (16.9cM) and Hst7 (4.83 cM).
  
  Significant linkage to testis weight also mapped to mid-chromosome X between DXMit119 (29.5 cM) and DXMit131 (59 cM). This locus accounts for 17% of the variance in testis weight and is designated Mhysq2 (male hybrid sterility QTL 2). Two linkage peaks appear within this interval; between DXMit119 and DXMit16 (LRS=13.5), and between DXMit16 and DXMit131 (LRS=13.6). Suggestive linkage to sperm count (LRS=7.5) was also observed at Mhysq2, explaining 10% of the genetic variance for this trait. Apreviously study by Storchova et al, 2004 also mapped low sperm count and testis weight to an interval on chromosome X between 50.5 cM and 58 cM in a cross involving PWD/Ph and C57BL/6J.
  
  Significant interactions were detected between Mhysq1 and Mhysq2 affecting sperm count (LRS=131), testis weight (LRS=103.7) and epididymis weight (LRS=94.8). Mhysq1 also displayed interaction with D10Mit75 (2 cM) on chromosome 10 for sperm count (LRS=114.1 cM) and testis weight (LRS=93.9), D10Mit55 (25.5 cM) for epididymis weight(LRS=90.6), and D18Mit149 (24 cM) on chromosome 18 for testis weight (LRS=92.9). These interacting loci are named Mhysq3 at D10Mit75, Mhysq4 at D10Mit55, and Mhysq5 at D18Mit149, respectively
  
  Suggestive linkage to sperm count (LRS=7.1) and epididymis weight (LRS=8.5) mapped to distal mouse Chromosome 3 near D3Mit57 (55 cM).