Mapping Data

Experiment

• Experiment
  TEXT-Cytogenetic Localization
• Chromosome
  18
• Reference
  J:6459 Francke U, et al., Chromosome assignment of a murine glucocorticoid receptor gene (Grl-1) using intraspecies somatic cell hybrids. Cell. 1980 Dec;22(3):657-64
• ID
  MGI:38773

Genes

Gene
Nr3c1

Notes

• Experiment
  Intraspecific somatic cell hybrids were constructed between mouse lymphoma lines S49.1 and EL4. Cell line S49.1 is glucocorticoid sensitive; cell line EL4 is glucocorticoid resistant. Unselected S49.1 x EL4 hybrids are glucocorticoid sensitive if they retain the complete chromosome complement of the parental lines, including the S49.1 specific glucocorticoid receptor (Nr3c1). Selection of S49.1 x EL4 hybrids for dexamethasone resistance results in specific loss of one Chromosome 18 (18¹) from the S49.1parent. These results suggest that in S49.1, one Chromosome 18 carries the wild type Nr3c1 gene and the other a mutant Nr3c1 that does not confer glucocortoid sensitivity. Nr3c1 is assigned to Chromosome 18.