Mapping Data

Experiment

• Experiment
  TEXT-Genetic Cross
• Chromosome
  17
• Reference
  J:8839 Rappold GA, et al., Identification of a testis-specific gene from the mouse t-complex next to a CpG-rich island. EMBO J. 1987 Jul;6(7):1975-80
• ID
  MGI:39024

Genes

Gene	Assay Type	Description
Tcd3	reported elsewhere
Dynlt2a1	Southern analysis	cosmid 117/11 fragment and probe 117B
Hba-ps4	reported elsewhere

Notes

• Experiment
  A t-complex gene (Tcte3) present in cosmid 117/11 was identified by analyzing sequences neighboring rare-cutter restriction sites that characterize CpG islands. These sequences were tested for transcription in mouse tissues and used to isolate a homologous cDNA clone. Tcte3 was mapped proximal to Hba-ps4 using a M. musculus x M. spretus backcross panel where Tcte3 segregation was followed using a cos 117/11 fragment restriction fragment length polymorphism (data not shown). Tcte3 was placed between the breakpoints of t^h49 and t³ using a restriction fragment length variant detected by probe 117B in a panel of partial t-haplotypes. Probe 117B derives from cos 117/8 which overlaps cos 117/11 by 10kb. Together these data map Tcte3 to a ~ 1 cM region in the central portion of the t-complex near Tcd3.