Mapping Data

Experiment

• Experiment
  CROSS
• Chromosome
  3
• Reference
  J:10048 Buchberg AM, et al., Localization of the murine macrophage colony-stimulating factor gene to chromosome 3 using interspecific backcross analysis. Genomics. 1989 Aug;5(2):363-7
• ID
  MGI:39658

Genes

Gene	Assay Type	Description
Mecom	Southern analysis	pUC1.OHP
Nras	Southern analysis	pSP6-NRAS
Ngf	Southern analysis	pmngf6
Csf1	Southern analysis	pUS-1
Amy2a5	Southern analysis	probe 3
Egf	Southern analysis	pmefg10b

Notes

• Experiment
  The total number of N2 progeny typed was 198, of these 40 were not typed for Evi-1. Those mice not tested for Evi-1 included 34 nonrecombinants, one recombinant between Csfm and Amy-2 and 5 recombinants between Amy-2 and Egf.Offspring types indicate alleles inherited from F1 parent.
  

CROSS

• Type
  Backcross, female
• Female Parent
  <b> <b> <b> <b> <b> <b>/<s> <s> <s> <s> <s> <s>
• Strain
  (C57BL/6J x M. spretus)F1
• Male Parent
  <b> <b> <b> <b> <b> <b>/<b> <b> <b> <b> <b> <b>
• Strain
  C57BL/6J
• Mapping Panel
  Copeland-Jenkins
• Allele 1
  b from C57BL/6J
• Allele 2
  s from M. spretus

CROSS Data

MC #mice	Mecom	Nras	Ngf	Csf1	Amy2a5	Egf
44	b	b	b	b	b	b
30	s	s	s	s	s	s
30	b	s	s	s	s	s
31	s	b	b	b	b	b
3	b	b	b	s	s	s
1	s	s	s	b	b	b
1	b	b	b	b	s	s
1	s	s	s	s	b	b
4	b	b	b	b	b	s
8	s	s	s	s	s	b
1	b	s	s	s	s	b
3	s	b	b	b	b	s
1	s	b	b	s	s	s

Statistics

Marker 1	Marker 2	# Recombinants	Total	% Recombinants	Std Error
Mecom	Nras	66	158	41.772	3.924
Nras	Ngf	0	158	0.000	0.000
Ngf	Csf1	5	158	3.165	1.393
Csf1	Amy2a5	2	158	1.266	0.889
Amy2a5	Egf	16	158	10.127	2.400