Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  3
• Reference
  J:10478 Kingsmore SF, et al., Long-range restriction site mapping of a syntenic segment conserved between human chromosome 1 and mouse chromosome 3. Genomics. 1990 May;7(1):75-83
• ID
  MGI:40153

Genes

Gene	Assay Type	Description
Cd2	Southern analysis	MT11.4
Atp1a1	Southern analysis	z4A
Ngf	Southern analysis	pmngf6
Tshb	Southern analysis	pTSHb
Ampd1	Southern analysis	rat Ampd-1 cDNA

Notes

• Reference
  Revised gene symbol: Cd2 (formerly Ly-37).
• Experiment
  Genomic DNA from C3H/HeJ-gld/gld mice was digested with rare cutting enzymes to physically map Cd2, Atp1a1, Ngfb, Tshb and Ampd1 using pulsed-field gel electrophoresis (PFGE). The following minimum fragment sizes were detected: Cd2 and Atp1a1 were detected on a 580kb NaeI band; Ngfb, Tshb, and Ampd1 were detected on 950 and 1250kb SacII bands; Cd2, Atp1a1, and Ngfb were detected on a 2300kb NruI band; Ngfb and Tshb were detected on 290 and 580kb BssHII bands; and Tshb and Ampd1 were on a 410kb SacII band. The gene order Cd2 - Atp1a1 - Ngfb - Tshb - Ampd1 was established with a maximum length for the Cd2 - Ampd1 interval of 2130kb.