Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  10
• Reference
  J:3245 MacDonald G, et al., Fine structure physical mapping of the region of mouse chromosome 10 homologous to human chromosome 21. Genomics. 1991 Oct;11(2):317-23
• ID
  MGI:40401

Genes

Gene	Assay Type	Description
Col6a1	Southern analysis	human Col6a-1 cDNA
Col6a2	Southern analysis	human Col6a-2 cDNA
Itgb2	Southern analysis	human Mp-9 cDNA
S100b	Southern analysis	S100b rat cDNA
Pfkl	Southern analysis	human Pfkl cDNA

Notes

• Experiment
  Physical mapping using pulse field gel electrophoresis was used to co-localize genes Pfkl, Itgb2, Col6a1, Col6a2 and S100b to a common region encompassing approximately 1900 kb of Chromosome 10. This region consists of one ordered segment Col6a1, Col6a2,S100b and a second segment consisting of Pfkl and Itgb2. Genomic DNA was prepared from A9 fibroblasts and C57BL/6J spleens. Enzymes used were NotI, SfiI, BassHII, SalI, EagI, MluI and XhoI.