Mapping Data

Experiment

• Experiment
  TEXT-Cytogenetic Localization
• Chromosome
  17
• Reference
  J:9988 Lader E, et al., tctex-1: a candidate gene family for a mouse t complex sterility locus. Cell. 1989 Sep 8;58(5):969-79
• ID
  MGI:41919

Genes

Gene	Assay Type	Description
D17Leh48	reported elsewhere
Dynlt1b	Southern analysis	c5-1
Tcp1	reported elsewhere	Fox, et al., Cell 40:63-69, 1985

Notes

• Reference
  Authors use T48 for D17Leh48.
• Experiment
  Dynlt1 was mapped to the t-complex region of Chromosome 17 distal to D17Leh48 and proximal to Tcp1 as follows. Somatic cell hybrid R-41, a Chinese hamster x C3H mouse hybrid carrying only mouse Chromosomes 17 and 18 was used to localize Dynlt1 to one of these chromosomes. Dynlt1 was mapped to the t-complex using RFLPs between C3H-+/+, C3H-+/t^w5, C3H-+/t^w32, and C3H-+/T t^sb tf. Seven independent proximal partial haplotype DNAs were shown to carry only the t copies of Dynlt1 thus mapping Dynlt1 to the proximal region of the mutant t-complex. Using the unequal recombinants t^ae5, t^w128, and t^w111, which all contain both wild-type and t-specific copies of Tcp1, Dynlt1 was shown to map proximal to Tcp1 in wild-type chromosomes. Using deletion mapping it was shown that wild-type Dynlt1 is present in the T^hp chromosome and deleted in the T^OR chromosome localizing Dynlt1 distal to D17Leh48.