Mapping Data

Experiment

• Experiment
  TEXT-Cytogenetic Localization
• Chromosome
  11
• Reference
  J:4100 Morris DJ, et al., Somatic cell hybrid mapping on mouse chromosome 11 (MMU11): assignment of markers relative to two breakpoints in band D. Genomics. 1993 Feb;15(2):323-31
• ID
  MGI:43018

Genes

Gene	Assay Type	Description
Egfr	Southern analysis	erbb
Hba	Southern analysis	murine alpha-globin
Il3	Southern analysis	pILM21
Sparc	Southern analysis	pC33.1
Polr2a	Southern analysis	pHRpII5.5
Pfn1	Southern analysis	HP1.0
D11H17S28	Southern analysis	pTB7
Trp53	PCR amplified length variant
Crk	Southern analysis	pCRK-1
Slc2a4	Southern analysis	phJHT-3
Ccl2	Southern analysis	pSP65
Mpo	Southern analysis	pMPO62
T(11;19)42H	karyotype
Hoxb	Southern analysis	MO-4.1
Col1a1	Southern analysis	pgIH-I
Rara	Southern analysis	p63
Phb1	Southern analysis	pHG7Bg4
Thra	Southern analysis	pE2A3D
Rnu1a1	Southern analysis	pUE236
D11Pas1	PCR amplified length variant
Gfap	PCR amplified length variant
Myl4	PCR amplified length variant
Slc4a1	PCR amplified length variant
Gh	Southern analysis	pcH800
D11Mit13	PCR amplified length variant
D11Mit11	PCR amplified length variant
D11Mit12	PCR amplified length variant
T(9;11)9Ad	karyotype
Gaa	Southern analysis	pHAGII
Tk1	Southern analysis
D11Jkn1e	Southern analysis	pIJ0.8
P4hb	Southern analysis	pHTB

Notes

• Reference
  Authors use Crk, D17s28h and Pfn for Crko, D11H17S28 and Pfn1 respectively.
• Experiment
  Somatic cell hybrids were constructed by fusion of mouse cells from translocation stocks T(11;19)42H and T(9;11)9Ad (T42H and T9Ad, respectively) with rat cells deficient in Tk1. Two hybrid clones were chosen for analysis - T42H4B and T42H5B. The data (not shown) provides mapping of loci either proximal or distal to the translocation breakpoints. (If a specific chromosome band was provided, it is in parenthesis following the locus). The loci which mapped proximal to the T42H breakpoint are Egfr (A1-A4) -Hba (A) - Il3 (A5/B1) - Sparc (B1) - Polr2a (B1/C) - Pfn1 - D11H17S28 (pter-D) - Trp53 (B4/B5) - Crk (pter-D) - Glut4 - Ccl2 - Mpo (C/E1). Following are the loci which mapped distal to the T42H breakpoint but proximal to the T9Ad breakpoint. Hox2 (D/E)- Col1a1 (D) - Rara (D) - Phb (D) - Thra (D/E) - Rnu1a1 (D/E) - D11Pas1 - Gfap - Myla (E) - Ae1 - Gh (distal) - D11Mit11 (distal) - D11Mit12 (distal). The following loci were mapped distal to the T9Ad breakpoint. Gaa - Tk1 (D/E) - D11Jnk1 - P4hb.