Mapping Data

Experiment

• Experiment
  TEXT-Congenic
• Chromosome
  1
• Reference
  J:152405 Kurey I, et al., Distinct genetic control of parasite elimination, dissemination, and disease after Leishmania major infection. Immunogenetics. 2009 Sep;61(9):619-33
• ID
  MGI:4360544

Genes

Gene	Assay Type
Lmr20	resistance/susceptibility
Lmr20a	resistance/susceptibility
Lmr20b	resistance/susceptibility
Lmr20c	resistance/susceptibility
D1Mit403	PCR amplified length variant

Notes

• Experiment
  In the present study the genetics of disease symptoms, immunological parameters and parasite load in draining lymph nodes and in the spleen following Leishmania major infection were analyzed.
  
  A special tool for the genetic analysis of multigenic traits, recombinant congenic strains, were used. Each of the 20 CcS/Dem RC mouse strains carries a different random subset of 12.5% genes of the resistant donor strain STS/A (STS) on the background of the susceptible strain BALB/cHeA (BALB/c). Two hundred and ninty nine (BALB/c x CcS11/Dem)F2 mice were used (155 were females and 144 were males). The CcS11 strain was in more than 38 generations of inbreeding.
  
  The CcS11 strain differs from BALB/c at STS-derived segments on eight chromosomes. These differential segements were typed in the F2 hybrid mice between BALB/c and CcS11 using 16 mircosatellite markers with a maximal interval between any two markers of 19 cM. Linkage analysis of the F2 hybids indicated four novel loci on Chromosomes 1, 7, 12, 16, and additional effects for a previoulsy detected locus on Chromosome 10.
  
  Lmr20, leishmaniasis resistance 20, mapped to Chr 1 of CcS11/Dem with marker D1Mit403 at 100.0 cM.
  
  03.30.2016 Curator Note: We have assigned distinct nomenclature to the individual disease phenotypes that mapped with significance to Lmr20, creating Lmr20a, Lmr20b and Lmr20c. Lmr20 represents the collection of individual traits analyzed that map to this same locus.
  
  Lmr20a was linked with marker D1Mit403 and it controls parasite load in draining lymph nodes, p=0.00554 and accounts for 4.24% of trait variance. BALB/c alleles at this locus were associated with higher parasite numbers. [Table 3.]
  
  Lmr20b was also linked with D1Mit403 and it controlled IgE serum concerntrations in female mice only, p=0.00181 and accounted for 11.76% of trait variance. BALB/c alleles were associated with higher IgE serum levels. [Table 5.]
  
  Lmr20c, also linked with D1Mit403, was detected in interaction with Lmr23 at D16Mit67. IFN gamma concentrations were influenced by this ineteraction, p=0.0201 and accounted for 6.93% of trait varaince. The highest level of IFN gamma was observed in STS/A homozygotes at both Lmr20c and Lmr23. [Table 6.]