Mapping Data

Experiment

• Experiment
  TEXT-Genetic Cross
• Chromosome
  5
• Reference
  J:19442 Nagle DL, et al., Structural analysis of chromosomal rearrangements associated with the developmental mutations Ph, W19H, and Rw on mouse chromosome 5. Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7237-41
• ID
  MGI:43682

Genes

Gene	Assay Type	Description
D5Buc1	Southern analysis	D5Buc1 probe1
D5Mnl25e	Southern analysis	D5Mnl25 cDNA1
D5Buc2	PFGE	D5Buc2 genomic1
Pdgfra	Southern analysis	Pdgfra probe2
D5Buc3	Southern analysis	D5Buc3 genomic1
Kit	Southern analysis	Kit probe2
Kdr	Southern analysis	Flk1 probe2
Ph	visible phenotype

Notes

• Experiment
  Deletion mapping of the F1 progeny from two interspecific crosses, C57BL/6J-Ph x M. spretus and C57BL/6J-W^19h x M. spretus, revealed that D5Buc1 maps outside the W^19h and Ph deletions; D5Mnl25, Kit and Flk1 are deleted in the W^19h but not the Ph chromosome while Pdgfra is deleted on both the W^19h and Ph chromosomes. One other locus, D5Buc3, maps within the Ph deletion but D5Buc2 maps outside the deletion. Pulse Field Gel Electrophoresis (PFGE) confirmed that D5Buc2 maps 50kb proximal to the Ph deletion breakpoint.