Mapping Data

Experiment

• Experiment
  TEXT-Cytogenetic Localization
• Chromosome
  5
• Reference
  J:19442 Nagle DL, et al., Structural analysis of chromosomal rearrangements associated with the developmental mutations Ph, W19H, and Rw on mouse chromosome 5. Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7237-41
• ID
  MGI:43683

Genes

Gene	Assay Type	Description
Pdgfra	FISH	ICRFy902G08127
D5Buc3	PFGE	D5Buc3 genomic1
Rw	visible phenotype
Kit	PFGE	Kit probe2

Notes

• Experiment
  Metaphase spreads of C57BL/6J-Rw lymphocytes were used to map Pdgfra by Fluorescent in situ Hybridization (FISH). The hybridization signal for Pdgfra differs on the two homologues of Chromosome 5 but the signal for Kit does not differ (see Stephenson, D.A. 1994, Mamm Genome) indicating that the breakpoint for the Rw inversion is located between Pdgfra and Kit. This was supported by Pulse Field Gel Electrophoresis (PFGE) data which indicate that the breakpoint was actually between D5Buc3 (a marker foundatthe most proximal end of the YAC clone containing Pdgfra) and Kit.