Mapping Data

Experiment

• Experiment
  CROSS
• Chromosome
  2
• Reference
  J:21512 Goldstein C, et al., Of mice and Marfan: genetic linkage analyses of the fibrillin genes, Fbn1 and Fbn2, in the mouse genome. Mamm Genome. 1994 Nov;5(11):696-700
• ID
  MGI:44125

Genes

Gene	Assay Type	Description
D2Mit63	PCR amplified length variant	A1132
B2m	Southern analysis	g2B2mdIIIB
Fbn1	Southern analysis	MF-13
Il1a	Southern analysis	pIL 1301
D2Mit19	PCR amplified length variant	A83

Notes

• Reference
  The authors suggest that Fbn1 and Fbn2 may be candidate genes for the Tsk and pk mutations respectively.
• Experiment
  Offspring types indicate alleles inherited from F1 parent.
  

CROSS

• Type
  Backcross, female
• Female Parent
  <a> <a> <a> <a> <a>/<s> <s> <s> <s> <s>
• Strain
  (AEJ/Gn-a Gdf5/a Gdf5 x M. spretus)F1
• Male Parent
  <a> <a> <a> <a> <a>/<a> <a> <a> <a> <a>
• Strain
  AEJ/Gn-a Gdf5
• Allele 1
  a from AEJ/Gn-a Gdf5
• Allele 2
  s from M. spretus

CROSS Data

MC #mice	D2Mit63	B2m	Fbn1	Il1a	D2Mit19
24	a	a	a	a	a
48	s	s	s	s	s
2	a	s	s	s	s
1	s	a	a	a	a
1	a	a	s	s	s
0	s	s	a	a	a
1	a	a	a	s	s
0	s	s	s	a	a
1	a	a	a	a	s
2	s	s	s	s	a

Additional Mice Typed Only for Specific Pairs of Loci

Marker 1	Marker 2	# Recombinants	# Parentals
D2Mit63	B2m	0	2
B2m	Fbn1	4	73
Fbn1	Il1a	1	78
Il1a	D2Mit19	0	12

Statistics

Marker 1	Marker 2	# Recombinants	Total	% Recombinants	Std Error
D2Mit63	B2m	3	82	3.659	2.073
B2m	Fbn1	5	157	3.185	1.401
Fbn1	Il1a	2	159	1.258	0.884
Il1a	D2Mit19	3	92	3.261	1.852