Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  18
• Reference
  J:21369 Luongo C, et al., Loss of Apc+ in intestinal adenomas from Min mice. Cancer Res. 1994 Nov 15;54(22):5947-52
• ID
  MGI:44482

Genes

Gene	Assay Type	Description
D18Mit19	PCR amplified length variant	A685
D18Mit20	PCR amplified length variant	B427
Apc	PCR amplified length variant	MAPCHIII-F, MAPCHIII-R
D18Mit24	PCR amplified length variant	J23
D18Mit33	PCR amplified length variant	B316
D18Mit4	PCR amplified length variant	M51

Notes

• Experiment
  Authors tested 97 adenomas (47 from C57BL/6-Min mice and 50 from AKR x C57BL/6-Min mice) for the presence of both Apc alleles - the normal and the Min allele. Without exception, the adenomas showed the loss of the normal Apc allele. Authors then tested in the F1 hybrid (AKR x C57BL/6-Min) 5 SSLP markers on Chromosome 18 (D18Mit19, D18Mit20, D18Mit24, D18Mit33, D18Mit4), which surround the Apc locus for the presence of the normal (carried by the AKR strain) or Min allele (carried by the C57BL/6 strain).All adenomas showed the loss of the AKR allele for the SSLP markers, indicating that Chromsome 18 was involved in the loss of the normal Apc allele.