Mapping Data

Experiment

• Experiment
  CROSS
• Chromosome
  8
• Reference
  J:24436 Naggert JK, et al., Hyperproinsulinaemia in obese fat/fat mice associated with a carboxypeptidase E mutation which reduces enzyme activity. Nat Genet. 1995 Jun;10(2):135-42
• ID
  MGI:45142

Genes

Gene	Allele	Assay Type	Description
D8Mit69		PCR amplified length variant	MPC1004
Cpe	Cpe^fat	direct sequencing	Cpe genomic1 and visible
D8Mit131		PCR amplified length variant	MT661
D8Mit78		PCR amplified length variant	MPC427
D8Mit50		PCR amplified length variant	B730
Ces1c		electrophoretic

Notes

• Reference
  Authors present evidence that the fat phenotype is encoded at the Cpe locus on Chromsome 8.
• Experiment
  The fat locus arose on a HRS/J background and was backcrossed onto the C57BLKS/J strain. Because these strains are unrelated, crossovers within the breeding colonies could be determined.

CROSS

• Type
  Intercross
• Female Parent
  <b> <b> <b> <b> <b> <b>/<h> <h> <h> <h> <h> <h>
• Strain
  C57BLKS/J-Cpe
• Male Parent
  <b> <b> <b> <b> <b> <b>/<h> <h> <h> <h> <h> <h>
• Strain
  C57BLKS/J-Cpe
• Allele 1
  b from C57BLKS/J
• Allele 2
  h from HRS/J

2x2 Data Reported

Marker 1	Marker 2	# Recombinants	# Parentals
D8Mit69	Cpe	17	383
Cpe	D8Mit131	7	393
D8Mit131	D8Mit78	39	361
D8Mit78	D8Mit50	18	382
D8Mit50	Ces1c	16	140