Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  5
• Reference
  J:29686 Nagle DL, et al., Physical mapping of the Tec and Gabrb1 loci reveals that the Wsh mutation on mouse chromosome 5 is associated with an inversion. Hum Mol Genet. 1995 Nov;4(11):2073-9
• ID
  MGI:45732

Genes

Gene	Allele	Assay Type	Description
Gabrb1		PFGE	Gabrb1 cDNA
Tec		Southern analysis	Tec cDNA
Kit	Kit^W-19H	PFGE	Gabrb1 cDNA, Tec cDNA

Notes

• Experiment
  PFGE analysis of large DNA fragments detected by the Gabrb1 cDNA and Tec cDNA probes in C57BL/6J, C3H/HeJ, and C57BL/6J-W^19H revealed altered restriction patterns associated with changes in chromosomal structure. Tec cDNA detected changed fragments in C57BL/6J-W^19H digested with BssHII, MluI, and NotI, while Gabrb1 cDNA detected changed DNA fragments only in C57BL/6J-W^19HDNA digested with MluI and NotI. Since Gabrb1, Tec, and D5Mnl25 have been shown to be contained on common MluI and NotI fragments and the BssHII fragment detected by Tec cDNA and not Gabrb1 cDNA is changed in C57BL/6J-W^19HDNA, the proximal breakpoint of the C57BL/6J-W^19H deletion (Kit-W-19h) was physically mapped between Tec and D5Mnl25.