Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  5
• Reference
  J:29686 Nagle DL, et al., Physical mapping of the Tec and Gabrb1 loci reveals that the Wsh mutation on mouse chromosome 5 is associated with an inversion. Hum Mol Genet. 1995 Nov;4(11):2073-9
• ID
  MGI:45733

Genes

Gene	Allele	Assay Type	Description
Gabrb1		PFGE	Gabrb1 cDNA
Pdgfra		reported elsewhere
D5Mnl25e		PFGE	D5Mnl25 probe1
Tec		PFGE	Tec cDNA
Kit	Kit^W-sh	PFGE	Kit probe1

Notes

• Experiment
  The authors performed PFGE analysis on Stock-W^sh/W^sh chromosomal DNA to physically map Kit^W-sh. Hybridization of Pdgfra probe3, Gabrb1 cDNA, Tec cDNA, and D5Mnl25 probe1 probes to Stock-W^sh/W^sh genomic DNA revealed altered fragments in DNA digested with several restriction enzymes. Additionally probes coresponding to loci detected at a considerable distance from each other detected altered DNA fragments of the same size. These results indicated that the Kit^W-sh mutation is likely to be achromosomal inversion spanning approximately 2.8 Mb and bringing together on the same large DNA fragments Gabrb1 and Pdgfra at the proximal side and Tec and Kit at the distal end, giving the following gene order: Gabrb1, Pdgfra, D5Mnl25, Tec, Kit.