Mapping Data

Experiment

• Experiment
  TEXT-Genetic Cross
• Chromosome
  17
• Reference
  J:31802 Gregorova S, et al., Sub-milliMorgan map of the proximal part of mouse Chromosome 17 including the hybrid sterility 1 gene. Mamm Genome. 1996 Feb;7(2):107-13
• ID
  MGI:46296

Genes

Gene	Assay Type	Description
T	visible phenotype
D17Leh119I	Southern analysis	p119A-R
D17Leh119II	Southern analysis	p119A-R
D17Mit18	PCR amplified length variant
D17Mit143	PCR amplified length variant
D17Mit164	PCR amplified length variant
Tcp1	PCR amplified length variant
Map3k4	Southern analysis	pMK174
Sod2	Southern analysis	pmMnSOD1
D17Mit57	PCR amplified length variant
D17Mit113	PCR amplified length variant
D17Ph2	restriction analysis	pC079R
D17Ph5e	restriction analysis	pc417
D17Mit78	PCR amplified length variant
D17Zt6	PCR amplified length variant
D17Zt7	PCR amplified length variant
Hst1	visible phenotype
Tbp	PCR amplified length variant
D17Ph4e	restriction analysis	pc891
D17Ph1	restriction analysis	pFFEH11R
D17Mit165	PCR amplified length variant
D17Leh80	PCR amplified length variant
D17Tu50	PCR amplified length variant
D17Mit59	PCR amplified length variant
D17Ph3	restriction analysis	pM10.1
D17Mit133	PCR amplified length variant
D17Mit26	PCR amplified length variant
D17Mit43	PCR amplified length variant
D17Mit46	PCR amplified length variant
D17Mit100	PCR amplified length variant
D17Mit173	PCR amplified length variant
Hba-ps4	Southern analysis	[a][g]4
D17Mit30	PCR amplified length variant
D17Mit29	PCR amplified length variant
D17Mit167	PCR amplified length variant
Pim1	Southern analysis	pPim1
D17Mit23	PCR amplified length variant
D17Mit45	PCR amplified length variant
D17Mit101	PCR amplified length variant
D17Mit61	PCR amplified length variant
H2-K1	Southern analysis	p1954
D17Mit62	PCR amplified length variant

Notes

• Reference
  MGD accession numbers for the backcross data and for physical mapping data are MGD-CREX-385 and MGD-PMEX-8 respectively. Tbp and D17Ph4e were proposed by the authors to be prime candidates for Hst1 based on their expression in the testes and complete linkage with Hst1. Authors use D17Leh119, this locus symbol has been split into two symbols D17Leh119I and D17Leh119II.
• Experiment
  To map the Hst1 locus, the progeny of a (C57BL/10-T/SnPh x C3H/DiPh)F1 x C57BL/10-T/SnPh backcross were analyzed. The presence of T mutation was scored by its short-tail phenotype, and H2-K was done by a microcytotoxicity test, and Southern analysis. Progeny from this cross was further analyzed by RFLP (Restriction Fragment Lenght Polymorphism) using loci D17Le119I, D17Leh119II, D17Rp17e, Tcp1, Sod2, D17Leh80, D17Tu50, Hba-ps4, Pim1 and H2-K. The order of loci were reported as: Centromere - 4.0 cM - D17Leh119I - D17Leh119II - D17Mit18 - D17Mit143 - D17Mit164 - 2.71 cM - D17Rp17e - 0.14 cM - Sod2 - D17Mit57 - D17Mit133 - 0.36 cM - D17Ph2 - D17Ph5e - D17Mit78 - D17Zt6 - D17Zt7 - 0.28 cM - Hst1 - Tbp - D17Ph4e - 0.14 cM - D17Ph1 - 0.86 cM - D17Mit165 - D17Leh80 - 0.21 cM - D17Tu50 - D17Mit59 - 1.71 cM - D17Ph3 - D17Mit133 - 0.43 cM - D17Mit26 - D17Mit43 - D17Mit46 - 0.29 cM - D17Mit100 - D17Mit173 - 0.36 cM - Hba-ps4 - 0.93 cM - D17Mit30 - 0.86 cM - D17Mit29 - 0.50 cM - D17Mit167 - 0.43 cM - Pim1 - D17Mit23- D17Mit45 - D17Mit101 - 0.07 cM - D17Mit61 - 2.72 cM - H2-K - D17Mit62.