Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  1
• Reference
  J:33172 Yui MA, et al., Production of congenic mouse strains carrying NOD-derived diabetogenic genetic intervals: an approach for the genetic dissection of complex traits. Mamm Genome. 1996 May;7(5):331-4
• ID
  MGI:46666

Genes

Gene	Assay Type
Idd5	resistance/susceptibility
D1Mit4	PCR amplified length variant
D1Mit5.1	PCR amplified length variant
D1Mit8	PCR amplified length variant
Bcl2	reported elsewhere
D1Mit15	PCR amplified length variant

Notes

• Experiment
  Microsatellite sequences were used as co-dominant markers to identify/map NOD mouse intervals containing recessive susceptibility alleles of insulin-related diabetes-associated genes. These intervals were transferred to C57BL/6, a resistant strain background, to produce 6 congenic strains. A gain of phenotype strategy was used in their construction.
  Linkage analysis identified 2 strong insulitis susceptibily genes on mouse Chromosome 1. A weak diabetes susceptibility locus found in another study was notfound by this method. The authors, anticipating that multiple loci relevant to IDD (insulin-dependent diabetes) and/or insulitis susceptibility are present on Chromosome 1, divided the entire linkage group into two overlapping intervals, each containing the diabetes peak and one insulin peak. Congenic strain B6.NODc1c mice contain the centromeric end of interval Idd5, including markers (in order) D1Mit4 - D1Mit5 - D1Mit8 - Bcl2 over an interval of 44 cM on mouse Chromosome 1. B6.c1t mice conntain the tleomeric end of Idd5, including markers D1Mit5 - D1Mit8 - Bcl2 - D1Mit15 - D1Mit17 over an interval of 47 cM on mouse Chromosome 1. The overall order of loci in the Idd5 region was reported as D1Mit4 - D1Mit5 - D1Mit8 - Bcl2 - D1Mit15.