Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  12
• Reference
  J:23551 Pennell CA, et al., Organization and expression of VH gene families preferentially expressed by Ly-1+ (CD5) B cells. Eur J Immunol. 1989 Nov;19(11):2115-21
• ID
  MGI:46687

Genes

Gene	Assay Type	Description
Igh-VJ558	reported elsewhere
Igh-V3609	reported elsewhere
Igh-VJ606	reported elsewhere
Igh-V12	Southern analysis	CH27VHPCR90-5
Igh-V3660	reported elsewhere
Igh-VGAM3.8	reported elsewhere
Igh-VS107	reported elsewhere
Igh-V11	Southern analysis	CH34VHPCR3.3
Igh-VX24	reported elsewhere
Igh-VQ52	reported elsewhere
Igh-V7183	reported elsewhere
Igh-D	reported elsewhere
Igh-J	reported elsewhere
Igh-C	reported elsewhere

Notes

• Experiment
  CXXB cell lines were generated by transforming BM (Bromelain-treated mouse erythrocytes) cells from 8-week old (BALB/cAnn.xid x C57BL/10J)F1 mice with Abelson murine leukemia virus. Combined deletion mapping profiles made by digesting CXXB line DNA with EcoRI (probed with CH34VHPCR3.3 for Igh-V11) and XbaI (probed with CH27VHPCR90-5 for Igh-V12) defined the Igh variable region. Results showed two Igh-V11 haplotype genes 3' of genes in the Igh-VGAM3.8 family and 5' of two Igh-VS107 genes. A single Igh-V12gene mapped 3' to some genes of the Igh-VJ505 family and 5' to genes of the entire IGH-VH3660 family. The overall order of genes was reported on mouse Chromosome 12 as: Igh-VJ558 - Igh-V3609 - Igh-V606 - Igh-V12 - Igh-V3660 - Igh-VGAM3.8 - Igh-V3660 - Igh-VS107 - Igh-VGAM3.8 - Igh-V11 - Igh-V3660 - Igh-V11 - Igh-VX24 - Igh-VS107 - Igh-VQ52 - Igh-V7183 - Igh-D - Igh-J - Igh-C where repeats of genes represent the location of various haplotypes (family genes).