Mapping Data

Experiment

• Experiment
  TEXT-Genetic Cross
• Chromosome
  5
• Reference
  J:32664 Okazaki Y, et al., Direct detection and isolation of restriction landmark genomic scanning (RLGS) spot DNA markers tightly linked to a specific trait by using the RLGS spot-bombing method. Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5610-4
• ID
  MGI:46831

Genes

Gene	Assay Type	Description
D5Ncvs18	RLGS	D5Ncvs18 probe1
D5Ncvs63	RLGS	D5Ncvs63 probe1
D5Ncvs64	RLGS	D5Ncvs64 probe1
D5Mit27	PCR amplified length variant	MUSGUSB
Gusb	PCR amplified length variant	MUSGUSB
D5Ncvs65	RLGS	D5Ncvs65 probe1
D5Ncvs19	RLGS	D5Ncvs19 probe1
D5Ncvs20	RLGS	D5Ncvs20 probe1

Notes

• Reference
  Authors use 5ht5a and rl for locus symbols Htr5a and Reln respectively.
• Experiment
  Congenic strain C57BL/6-Gus^s, which has the Gus region from M. spretus, was produced by nine generations of backcrossing and selection. Using the Restriction Landmark Genomic Scanning (RLGS) method (J22223 Okazaki (1994) Biochem Biophys Res Commun Dec 30;204(3):1992-9), the authors compared profiles of C57BL/6-Gus^s, M. spretus, and C57BL/6 mice around the Gus mutation region. A total of 10,565 loci were examined by using seven enzyme combinations. Of these, 33 loci were mapped to the Gus region, localizing the M. spretus contribution to a <4.2 cM congenic region. The following genetic order dtermined was as follows: D5Ncvs18 - D5Ncvs63 - D5Ncvs64 - D5Mit27 - Gus - D5Ncvs65 - D5Ncvs19 - D5Ncvs20.