Mapping Data

Experiment

• Experiment
  TEXT-Genetic Cross
• Chromosome
  5
• Reference
  J:32664 Okazaki Y, et al., Direct detection and isolation of restriction landmark genomic scanning (RLGS) spot DNA markers tightly linked to a specific trait by using the RLGS spot-bombing method. Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5610-4
• ID
  MGI:46853

Genes

Gene	Assay Type	Description
D5Mit47	PCR amplified length variant	MUSMDR
Abcb1b	PCR amplified length variant	MUSMDR
D5Rik87	RLGS
D5Rik88	RLGS
D5Rik89	RLGS
D5Rik90	RLGS
D5Rik91	RLGS
D5Rik92	RLGS
D5Rik93	RLGS
D5Rik94	RLGS
D5Mit1	PCR amplified length variant
D5Rik95	RLGS
D5Rik96	RLGS
D5Mit227	PCR amplified length variant
D5Rik97	RLGS
D5Rik98	RLGS
D5Rik99	RLGS
D5Mit61	PCR amplified length variant
Reln	visible phenotype
D5Mit72	PCR amplified length variant
D5Rik100	RLGS
D5Rik101	RLGS
D5Rik102	RLGS
D5Rik103	RLGS
D5Rik104	RLGS
D5Mit180	PCR amplified length variant
Htr5a	Southern analysis	Htr5a probe2

Notes

• Reference
  Authors use 5ht5a and rl for locus symbols Htr5a and Reln respectively.
• Experiment
  Forty eight backcross mice [(BALB/cA-rl/rl x M. spretus) F1 x BALB/cA-rl/rl] that had meiotic recombinations between D5Mit47 and Htr5a (5ht5a) were screened for 8856 Restriction Landmark Genomic Scanning (RLGS) loci using 4 enzyme combinations. Thirty one of these loci (designated D5Rik87-D5Rik117) were mapped to the region near the Reln locus by observation of relative spot intensity between homozygous (Reln^c, D5Mit47, Htr5a - full intensity) and heterozygous (half intensity) animals. D5Rik97, D5Rik98and D5Rik99 showed no recombination to Reln. The following locus order was reported: D5Mit47 - Pgy1 - D5Rik87 - D5Rik88 - D5Rik89 - D5Rik90 - D5Rik91 - D5Rik92 - D5Rik93 - D5Rik94 - 1.54 cM - D5Mit1 - D5Rik95 - D5Rik96 - D5Mit227 - 1.67 cM - D5Rik97 - D5Rik98 - D5Rik99 - D5Mit61 - Reln - D5Mit72 - D5Rik100 - D5Rik101 - D5Rik102 - D5Rik103 - D5Rik104 - D5Mit180.