Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  Y
• Reference
  J:34101 Elliott DJ, et al., An RBM homologue maps to the mouse Y chromosome and is expressed in germ cells. Hum Mol Genet. 1996 Jul;5(7):869-74
• ID
  MGI:46923

Genes

Gene	Assay Type	Description
Rbmy	PCR amplified length variant	g632, g747

Notes

• Reference
  Authors use Rbm for locus symbol Rbm1.
• Experiment
  Using primers specific for Rbm1, authors report testing DNA from male, female, XXSxr^a, and XXSxr^b mice by PCR amplification. All except the female DNA sample amplified, giving a product of the predicted size. The data maps one or more copies of Rbm1 within the Sxr^a region of the mouse Y Chromosome, and excludes the possibility that all copies of Rbm1 are within the region defined by the Sxr^b deletion. The authors stated that they were concerned that the use of PCR might be biasing the sequences that they were detecting because it had been suggested that there may be a number of closely related sequences in this region of mouse Chromosome Y. To examine this possibility, authors used either the pmRbm1 or the Rbm1 specific probe to probe SOuthern blots under low and high stringency conditions. Authors found that the probe detected a male specific 6kb fragment that was also present in XXSxr^a and XXSxr^b DNA samples. These results confirmed the previous findings using PCR amplification. It should be noted however, that authors also reportthat direct sequencing of the region defined by the g632/h295 PCR product coupled with the results of Southern blot analysis indicate that there are multiple copies of Rbm1 on mouse Choromsome Y.