Mapping Data

Experiment

• Experiment
  TEXT-Genetic Cross
• Chromosome
  17
• Reference
  J:32563 Khambata S, et al., Ea recombinational hot spot in the mouse major histocompatibility complex maps to the fourth intron of the Ea gene. Genome Res. 1996 Mar;6(3):195-201
• ID
  MGI:47199

Genes

Gene	Assay Type	Description
D17Rua10	PCR amplified length variant	10F, 10R
D17Rua2	PCR amplified length variant	D17Rua2-pA, D17Rua2-pB
H2-Ea	restriction analysis	H2-Ea genomic 4

Notes

• Reference
  Markers D17Rua10 and D17Rus2 are included within H2-Ea.
• Experiment
  Sets of PCR primers within the H2-Ea region of mouse Chromosome 17 were designed to include exon 5 (D17Rua10) and intron 2 (D17Rua2). The products of these primers were polymorphic for strains in a backcross. Recombination of D17Rua10 and D17Rua2 was observed in 7 of 750 backcross animals from 3 separate crosses. Restriction analysis of a genomic clone derived by PCR amplification within the region of recombination was used to show that a hotspot of recombination occurred over a 2.2kb region of the H2-Eagene and flanked by markers D17Rua10 and D17Rua2.