Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  13
• Reference
  J:33544 Perou CM, et al., Complementation of the beige mutation in cultured cells by episomally replicating murine yeast artificial chromosomes. Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5905-9
• ID
  MGI:47941

Genes

Gene	Allele	Assay Type	Description
Nid1		PCR amplified length variant	Nid-pA, NId-pB
D13Uth1		overlapping clones	195.A8, 151.H1, 195.A8-5'
D13Uth2		overlapping clones	C9.E7, C96.G11, 195.A8, 151.H1, C9.E7-3'
D13Uth3		overlapping clones	C9.E7, C96.G11, 195.A8, 151.H1, 113.G6, 113.G6-3'
D13Uth4		overlapping clones	C9.E7, C96.G11, 195.A8, 151.H1, 113.G6, C9.E7-5'
D13Uth5		overlapping clones	195.A8, 113.G6, 195.A8-3'
Lyst	Lyst^bg	visible phenotype

Notes

• Experiment
  A YAC complementation strategy was used to localize the Lyst^bg locus in mice. A series of overlapping YACs (isolated by PCR screening for Nid sequences, previously shown to be near Lyst^bg on Mouse Chromosome 13) were introduced to bg mouse fibroblasts by spheroplast fusion. Fusions were screened for phenotypically small lysosomes no longer clustered in the perinuclear region and the disappearance of tubular lysosomes, indicating bg complementation. Complementary YACs were isolated and partially sequenced. The YACs were physically mapped using STS PCR analysis. Locus Lyst^bg was localized to a 500kb region roughly 70kb from Nid. The following order of loci on mouse Chromosome 13 was reported: D13Uth1 - D13Uth2 - Nis - D13Uth3 - D13Uth4 - Lyst - D13Uth5.