Mapping Data

Experiment

• Experiment
  TEXT-Genetic Cross
• Chromosome
  11
• Reference
  J:36967 Sornson MW, et al., Pituitary lineage determination by the Prophet of Pit-1 homeodomain factor defective in Ames dwarfism. Nature. 1996 Nov 28;384(6607):327-33
• ID
  MGI:48267

Genes

Gene	Assay Type	Description
D11Rsd1	molecular technique	BsrG480
D11Rsd2	molecular technique	Bcl730c
Prop1	molecular technique	5E9 and 21F20
D11Rsd3	molecular technique	Bgl700
D11Rsd4	molecular technique	Nhe900

Notes

• Experiment
  Six probes identified by a subtractive hybridization technique (GDRDA) were mapped back to mouse Chromosome 11 in an intercross between (CAST/Ei x DF/B df/df)F1 mice. These probes were associated with loci that mapped with the Prop1 phenotype df and identified a genetic distance of approximately 1.7 Mb deriving an order of: D11Rds1 - D11Rds2 - Prop1 - D11Rds3 - D11Rds4. In addition, two of the probes were non-recombinant with Prop1. The genetic distance of 1.7 Mb was further refined to approximately 400-600kb by pulsed field genomic Southern analysis. These data were informative for obtaining BAC clones that carry Prop1 genomic segments.