Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  11
• Reference
  J:37668 Nabetani A, et al., Mouse U2af1-rs1 is a neomorphic imprinted gene. Mol Cell Biol. 1997 Feb;17(2):789-98
• ID
  MGI:48296

Genes

Gene	Assay Type	Description
Zrsr2-ps1	PFGE	NotI/EcoRV probe, SmaI/NotI probe
Commd1	PFGE	B-E, B-P, P-E

Notes

• Experiment
  Authors used pulse field gel electrophoresis in order to map U2af1-rs1 in relation to Murr1 on mouse Chromosome 11. Three kinds of Commd1 cDNA fragments, B-P (for the 5' region), P-E (for the 3' region), and B-E (for the central region), were used as probes in this analysis. The 5' and 3' probes hybridized with the 300kb and 120kb fragments respectively. The 300kb fragment was also detected by the U2af1-rs1 NotI/EcoRV probe, and the 120kb fragment by the U2af1-rs1 SmaI/NotI probe. Since U2af1-rs1 has oneNotI site and no introns, authors concluded that the Commd1 gene has an intron which contains the entire U2af1-rs1 gene.