Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  4
• Reference
  J:39477 Lander JK, et al., Low-frequency loss of heterozygosity in Moloney murine leukemia virus-induced tumors in BRAKF1/J mice. J Virol. 1997 May;71(5):3940-52
• ID
  MGI:48394

Genes

Gene	Assay Type	Description
D4Mit82	PCR amplified length variant
D4Mit27	PCR amplified length variant
D4Mit12	PCR amplified length variant
D4Mit69	PCR amplified length variant
D4Mit13	PCR amplified length variant
D4Mit62	PCR amplified length variant
Mpmv19	Southern analysis	JS-4
Pmv25	Southern analysis	JS-5
Cdkn2a	Southern analysis	Cdkn2a genomic1
Cdkn2b	Southern analysis	Cdkn2b genomic1

Notes

• Experiment
  F1 progeny derived from C57BR/cdJ x AKR/J mice were induced with MMLV and 52 tumor cell lines analyzed in a genome wide scan for LOH (loss of heterozygosity) of polymorphic endogenous viral loci. LOH of AKR/J contributed mouse Chromosome 4 loci Mpmv19 and Pmv25 was observed. The known locations of the viral insertions in mouse Chromosome 4 enabled the investigation of the LOH of polymorphic microsatellite markers within the region. Markers D4Mit82, D4Mit27, D4Mit12, D4Mit69, D4Mit13 and D4Mit62 spanning approximately 45 cM of distal mouse Chromosome 4 also showed a LOH in 3 tumor cell lines which was consistent with that observed for Mpmv19 and Pmv25 loci. Interestingly the region deleted in mouse Chromosome 4 shares conservation with a region of human Chromosome 9 that contains tumor suppressor loci, however the mouse homologues of these loci, Cdkn2a and Cdkn2b, were not within the region deleted in the F1 progeny.