Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  18
• Reference
  J:39174 Smits R, et al., Loss of Apc and the entire chromosome 18 but absence of mutations at the Ras and Tp53 genes in intestinal tumors from Apc1638N, a mouse model for Apc-driven carcinogenesis. Carcinogenesis. 1997 Feb;18(2):321-7
• ID
  MGI:48449

Genes

Gene	Assay Type	Description
D18Mit64	PCR amplified length variant
Apc	PCR amplified length variant	Apc-A2/Apc-C2, Apc-A2/Neo3
D18Mit58	PCR amplified length variant

Notes

• Experiment
  Authors note that a high number of tumors retained the 120/Ola alleles at several Chromosome 18 markers and that this allowed them to perform Chromosome 18 LOH analysis of all of the C57BL/6-Apc⁺/Apc^1638N tumors. Using mouse Chromosome 18 markers that are known to be polymorphic between 129/Ola and C57BL/6 inbred strains, authors tested 35 C57BL/6-Apc⁺/Apc^1638N tumors where the Apc gene was lost. D18Mit64 also showed a LOH. The marker D18Mit58 showed a LOH in 28 of the 29 tumors where Apc was lost. No LOH was observed for these markers without the detectable loss of the wildtype Apc gene. This data shows that mouse Chromosome 18 is involved in the loss of the wildtype Apc allele.