Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  6
• Reference
  J:37097 Jang W, et al., Localization of the rhotekin gene RTKN on the physical maps of mouse chromosome 6 and human chromosome 2p13 and exclusion as a candidate for mnd2 and LGMD2B. Genomics. 1997 Mar 15;40(3):506-7
• ID
  MGI:48537

Genes

Gene	Assay Type	Description
D6Mit70	overlapping clones	P1-2, P1-3
D6Mm1	overlapping clones	P1-2, P1-7
Rtkn	overlapping clones	P1-7
D6Mm2	overlapping clones	P1-7, P1-4
D6Mit128	overlapping clones	P1-4

Notes

• Experiment
  Authors identified a putative exon in P1-7 which is located within a P1 contig that includes the markers D6Mit70 and D6Mit128. Sequence comparison analysis identified this exon as belonging to Rtkn, Southern analysis was then done by hybridizing P1-7 anda Rtkn cDNA probe to NsiI digested 129/SvJ genomic DNA. P1-7 and the Rtkn cDNA probe gave the same hybridizing fragments, confirming that the entire Rtkn gene is located on the P1 clone, P1-7. These results physically mapped Rtkn to mouse Chromosome 6 inthe following gene order: D6Mit70 - D6Mm1 - Rtkn - D6Mm2 - D6Mit128.