Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  15
• Reference
  J:112668 Wittenburg H, et al., QTL mapping for genetic determinants of lipoprotein cholesterol levels in combined crosses of inbred mouse strains. J Lipid Res. 2006 Aug;47(8):1780-90
• ID
  MGI:5620434

Genes

Gene	Assay Type
Nhdlq9	susceptibility/resistance
D15Mit171

Notes

• Experiment
  To identify loci that influence lipo-protein cholesterol levels QTL mapping was performed on PERA/EiJ x I/LnJ and PERA/EiJ x DBA/2J intercrosses and in a combined data set from both crosses. Parental strain PERA/EiJ exhibits increased HDL cholesterol compared to I/LnJ and DBA/2J after 8 weeks on the high fat diet, while parental strain I/LnJ exhibits increased non-HDL cholesterol compared to PERA/EiJ and DBA/2J.
  
  LOD scores for HDL cholesterol concentrations were considered significant when exceeding 2.9in each of the PERA intercrosses. A LOD score of 3.0 or better was the threshold for significance in the combined cross data when the cross was an additive covariate; 3.7 when the cross was an interactive covariate. The analysis of combined data from bothcrosses increased the detection power of the analysis.
  
  Significant linkage to non-HDL cholesterol mapped to 56.0 cM near D15Mit171 in the (PERA/EiJ x DBA/2J)F2 cross, LOD=3.3. The LOD score increased to 4.0 when data from both crosses were combined and the cross was considered as an interactive covariate.This locus is named Nhdlq9 (non-HDL QTL 9). The QTL interval spans 44cM - 62 cM. DBA/2J-derived alleles at Nhdlq9 confer increased non-HDL cholesterol.