Mapping Data

Experiment

• Experiment
  TEXT-Meta Analysis
• Chromosome
  17
• Reference
  J:87796 Turri MG, et al., Multivariate analysis of quantitative trait loci influencing variation in anxiety-related behavior in laboratory mice. Mamm Genome. 2004 Feb;15(2):69-76
• ID
  MGI:5763651

Genes

Gene
Axtrb9

Notes

• Reference
  DeFries strains:
  QTL were mapped in two large F2 intercrosses from four lines derived from a replicated selection experiment for open-field activity, an animal model for susceptibility to anxiety. The foundation for the selection experiment consisted of an F3 generation derived from a cross between inbred lines, BALBc/J and C57BL/6J. Ten F3 litters that contained at least two males and females were chosen at random. The most active male and female within each litter were selected to become the progenitors of one selected line, termed H1. The least active male and female from each of the same ten litters were selected to serve as progenitors of a low activity line, L1. The same selection procedure was applied to members of a second randomly chosen group of 10 F3 litters as parents for replicate selected lines H2 and L2. The resulting progeny represented the first selected generation. Within the two high lines the most active male and female were selected from each litter, whereas the least active male and female were selected from each litter in the two low lines.Thirty generations of selection were completed and in generation 30 open field activity scores of the high lines were 30 times greater than those of the low lines. Following the suspension of selection the lines were randomly mated within line for 18 generations and then inbred using brother-sister mating.
  
• Experiment
  Multivariate approaches are used here to uncover the genetic architecture of anxiety related phenotypes. Novel QTL were detected on Chromosomes 3, 9, 13 and 17.
  
  Genotype and phenotype data generated from combined results from (H1 x L1) and (H2 x L2) intercrosses of 1636 F2 DeFries mice, in previously published works (J:70479 and J88525), were used. Genetic mapping data was analyyzed using MAPMAKER software. Multivariate analyses were performed with Multi-QTL and JZ-MAPQTL.
  
  Twenty-three phenotypes (Table 1) were analyzed jointly using Multi-QTL and obtained genome-wide significance threshold by permutation. Analysis detected LOD scores with p< 0.05 on 15 chromosomes (Table 2); 10 of which corresponded to those previoulsy identified by single-trait analysis on Chrs 1, 4, 7, 8, 11, 12, 14, 15, 18, and X. On 4 chromosomes, 3, 9, 13 and 17 only the multivariate analyses identified QTL.
  
  Axtrb9, anxiety-related behaviors 9, mapped to Chromosome 17 with a LOD=22.5, p=0.0000. [Table 2].
  Traits contributing to the Axtrb9 QTL were [Table 3]:
  open field defecation, p=0.019;
  activity in the center of the open field, p=0.002;
  time spent in the center of the open field, p=0.011;
  activity in the dark side of the light dark box, p=0.006;
  transitions in the light dark box, p=0.006;
  activity in the light side of the light dark box, p=0.010;
  time spent on the light side of the light dark box, p=0.004;
  latency in the light dark box, p=0.008;
  closed entries in the elevated plus maze, p=0.000;
  activity in the closed arm of the elevated plus maze, p=0.000; and
  latency to enter the open arm of the elevated plus maze, p=0.028