Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  10
• Reference
  J:170900 Concepcion D, et al., Modifier Genes for Mouse Phosphatidylinositol Transfer Protein {alpha} (vibrator) That Bypass Juvenile Lethality. Genetics. 2011 Apr;187(4):1185-91
• ID
  MGI:5790045

Genes

Gene	Assay Type
Mvb2	visible phenotype
Mvb4	visible phenotype

Notes

• Experiment
  The authors sought to identify major effect modifier loci for mouse phosphatidylinositol transfer protein a (vibrator) that bypass juvenile lethality. Linkage analysis was performed on 87 (B6.D2(Cg)-Pitpna^vb x A/J)F2-Pitpna^vb/Pitpna^vb animals using survival beyond 42 days (the maximum survival observed among B6.D2(Cg)-Pitpna^vb animals) as a dichotomous trait and using survival latency in days as a quantitative trait. Animals were drawn from two equivalent but independent crosses performed in 2003-2004 (81 vb/vb) and 2007-2008 (140 vb/vb), with similar phenotype distributions. The authors genotyped 45 long-lived (approx. 49 days) and 42 short-lived (approx 41 days) animals biased for extreme survival times and including all of the longest-lived. QTL were mapped using using microsatellite (SSLP) markers. Development of additional markers, to distinguish A/J and C57BL/6J alleles in a long segment of low divergence on chromosome 10, was guided by segments containing SNP polymorphisms in published maps (Pletcher et al. 2004). The authors identified three QTL reaching genome-wide significance:
  
  QTL Mvb2 (modifier of vibrator 2) is significant for the dichotomous trait of survival past 42 days. It maps to Chromosome 10 with a peak LOD score at D10Mit233. Mvb2 shows a semidominant survival effect with AA > AB > BB genotypes.
  
  QTL Mvb3 (modifier of vibrator 3) is significant for the dichotomous trait of survival past 42 days. It maps to Chromosome 3 with a peak LOD at D3Mit315. Mvb3 shows molecular heterosis with AB > AA or BB.
  
  QTL Mvb4 (modifier of vibrator 4) is significant for the quantitative trait of survival latency. It maps to Chromosome 10 with a peak LOD at D10Mit233. Mvb4 shows a semidominant survival effect witih AA > AB > BB genotypes.