Mapping Data

Experiment

• Experiment
  TEXT-Congenic
• Chromosome
  11
• Reference
  J:203042 Hoover-Plow J, et al., Genetic dissection of quantitative trait Loci for hemostasis and thrombosis on mouse chromosomes 11 and 5 using congenic and subcongenic strains. PLoS One. 2013;8(10):e77539
• ID
  MGI:5792120

Genes

Gene	Assay Type
Hmtb6	visible phenotype
D11Mit179	PCR amplification
D11Mit258	PCR amplification
D11Mit336	PCR amplification

Notes

• Experiment
  QTL for hemostasis and thrombosis, Hmtb4, Hmtb5 and Hmtb6, were previoulsy identified using F2 progeny from a cross of (C57BL/6J-Chr 11^A/J/NaJ [CSS11] x C57BL/6J) mice and a cross of (C57BL/6J-Chr 5^A/J/NaJ [CSS5] x C57BL/6J) mice in J:139651 [pmid:18787898].
  
  The goal of this study was to fine map the previoulsy identified QTL using congenic and subcongenic mouse strains.
  
  To verify the previously identified QTL Hmtb6, linked to clot stability time, congenic strains were constructed by crossing CSS11 and C57BL/6J mice to produce the congenic strain B6.A(D11Mit179-D11Mit336)/NaJ and subcongenic strain B6.A(D11Mit258-D11Mit336)/NaJ.
  
  Compared with C57BL/6J mice the subcongenic strain B6.A(D11Mit258-D11Mit336)/NaJ accounted for 100% of the increase in clot stability time for CSS11, where the congenic strain B6.A(D11Mit179-D11Mit336)/NaJ accounted for only 50% of the increase - suggesting that the trait was isolated in the D11Mit258 to D11Mit336 region and dominantly inherited. Hmtb6, p=0.05, mapped to a 2.9 Mbp region between 107.6-101.5 Mbp on distal Chr 11 where there are 25 protein coding genes. Genes in the A/J fragment were Abca5, Apoh, and Gna13.