Mapping Data

Experiment

• Experiment
  TEXT-QTL-Candidate Genes
• Chromosome
  12
• Reference
  J:240255 Kobayashi M, et al., Detection of differentially expressed candidate genes for a fatty liver QTL on mouse chromosome 12. BMC Genet. 2016 Jun 06;17(1):73
• ID
  MGI:5910539

Genes

Gene	Assay Type
Fl1sa	reported elsewhere
Iah1
Rrm2
Prkd1

Notes

• Experiment
  The SMXA-5 mouse is an animal model of high-fat diet-induced fatty liver. The major QTL for fatty liver, Fl1sa on chromosome 12, was previously identified in a SM/J SMXA-5 intercross. The SMXA-5 genome consists of the SM/J and A/J genomes, and the A/J allele of Fl1sa is the fatty liver-susceptibility allele. The existence of the responsible genes for fatty liver within Fl1sa was confirmed in A/J-Chr 12^SM/J consomic mice. [J:124974].
  
  The aim of the current study was to identify candidate genes for Fl1sa, and to investigate whether the identified genes affect the lipid metabolism.
  
  To identify candidate genes for Fl1sa, DNA microarray analysis was performed using the livers of A/J-Chr 12^SM/J and A/J mice that were fed a high-fat diet. The mRNA levels of three genes (Iah1, Rrm2, Prkd1) in the chromosomal region of Fl1sa were significantly different between the strains.
  
  Iah1 mRNA levels in the liver, kidney, and lung were significantly higher in
  A/J-Chr 12^SM/J mice than in A/J mice. The hepatic Iah1 mRNA level in A/J-Chr 12^SM/J mice was 3.2-fold higher than that in A/J mice. A stable cell line expressing the mouse Iah1 protein in mouse hepatoma Hepa1-6 cells was constructed. Overexpression of Iah1 in Hepa1-6 cells suppressed the mRNA levels of Cd36 and Dgat2, genes which play important roles in triglyceride synthesis and lipid metabolism.