Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  9
• Reference
  J:175701 Yang IV, et al., A locus on chromosome 9 is associated with differential response of 129S1/SvImJ and FVB/NJ strains of mice to systemic LPS. Mamm Genome. 2011 Oct;22(9-10):518-29
• ID
  MGI:6501591

Genes

Gene	Assay Type
Hrsl1	visible phenotype
Casp4	visible phenotype

Notes

• Experiment
  Although polymorphisms in TLR receptors and downstream signaling molecules affect the innate immune response, these variants account for only a portion of the ability of the host to respond to microorganisms. To identify novel genes that regulate the host response to systemic lipopolysaccharide (LPS), the authors created an F2 intercross between susceptible (FVB/NJ) and resistant (129S1/SvImJ) strains, challenged F2 progeny with LPS via intraperitoneal injection, and phenotyped 605 animals for survival and another 500 mice for serum concentrations of IL-1B and IL-6.
  
  Allele-specific genotyping by quantitative PCR (Germer and Higuchi 1999) was used to genotype pools of DNA from the most susceptible and resistant F2 mice at 300 SNP markers throughout the mouse genome. Genome-wide scans were performed on pools of susceptible and resistant mice for survival, IL-1b, and IL-6.
  
  This approach identified a single locus (genome coordinates relative to NCBI Build 37/mm9):
  
  Hrsl1 (host response to systemic lipopolysaccharide 1) maps to Chr 9: 0 - 40 Mb.
  
  Casp4 is suggested as the most likely candidate gene in this region.