Mapping Data

Experiment

• Experiment
  TEXT-QTL-Candidate Genes
• Chromosome
  3
• Reference
  J:313290 Starcher AE, et al., A systems approach using Diversity Outbred mice distinguishes the cardiovascular effects and genetics of circulating GDF11 from those of its homolog, myostatin. G3 (Bethesda). 2021 Sep 2;
• ID
  MGI:7286093

Genes

Gene	Assay Type
Gdfsc1	visible phenotype
Hey1	visible phenotype
Smc1	visible phenotype
Foxo1	visible phenotype

Notes

• Reference
  The Diversity Outbred heterogeneous stock (J:DO) is a developing mouse population derived from progenitor lines of the Collaborative Cross (CC). The CC is a panel of recombinant inbred (RI) mouse strains that combines the genomes of eight genetically diverse founder strains - A/J, C57BL/6J, 129S1/SvImJ, NOD/ShiLtJ, NZO/HlLtJ, CAST/EiJ, PWK/PhJ, and WSB/EiJ - to capture nearly 90% of the known variation present in laboratory mice (Churchill et al. 2004). Animals from 160 incipient CC lines at early stages of inbreeding were used to establish the DO population, which is maintained by a randomized outbreeding strategy that avoids brother-sister matings. The DO and CC populations thus capture the same set of natural allelic variants derived from a common set of eight founder strains, with DO mice being outbred and the CC population being inbred.
  
  CTC (2004), Churchill, G. A., et al. The Collaborative Cross, a community resource for the genetic analysis of complex traits. Nat Genet. 36, 1133-7.
• Experiment
  QTL Gdfsc1 (GDF11 serum concentration 1) maps to Chr 3: 3.039589 - 9.983782 Mb in close proximity to the gene Hey1, a transcriptional repressor. Bioinformatic analyses located putative binding sites for the HEY1 protein upstream of the Gdf11 gene in the mouse and human genomes. While these findings indirectly support Hey1 as a candidate gene behind circulating GDF11 levels, the authors also cannot exclude other plausible genes within the same locus, such as Stathmin-2 (Stmn2). Stmn2 is another reasonable candidate as it is expressed in both murine and human heart tissue throughout development and into adulthood (Asp et al. 2019; Bult et al. 2019; Smith et al. 2019).
  
  QTL Smc1 (serum myostatin concentration 1) maps to Chr 3: 52.26269 - 52.71985 Mb. The peak is located in close proximity to protein-coding gene Forkhead Box O1 (Foxo1; MGI:1890077).