Mid2 MGI Mouse Gene Detail - MGI:1344333

Symbol

Mid2
Name

midline 2
Synonyms

FXY2, Trim1

Feature Type

protein coding gene
IDs

MGI:1344333
NCBI Gene: 23947
Alliance

gene page
Transcription Start Sites

8 TSS

Sequence Map

ChrX:139565348-139668464 bp, + strand
From Ensembl annotation of GRCm39
View this region in JBrowse
Genome Browsers

Ensembl | UCSC | NCBI

Genetic Map

Chromosome X, 61.35 cM
Mapping Data

3 experiments

SNPs within 2kb

2053 from dbSNP Build 142
Strain Annotations

18
PCR

2

For selected strains:

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_1344333	protein coding gene	ChrX:139565326-139668464 (+)
129S1/SvImJ	MGP_129S1SvImJ_G0036136	protein coding gene	ChrX:138224560-138337594 (+)
A/J	MGP_AJ_G0036114	protein coding gene	ChrX:137599140-137711208 (+)
AKR/J	MGP_AKRJ_G0036039	protein coding gene	ChrX:142006530-142121196 (+)
BALB/cJ	MGP_BALBcJ_G0036104	protein coding gene	ChrX:135907265-136015838 (+)
C3H/HeJ	MGP_C3HHeJ_G0035813	protein coding gene	ChrX:138236245-138345212 (+)
C57BL/6NJ	MGP_C57BL6NJ_G0036629	protein coding gene	ChrX:142265639-142368306 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0033453	protein coding gene	ChrX:129318486-129416959 (+)
CAST/EiJ	MGP_CASTEiJ_G0035104	protein coding gene	ChrX:119057024-119159953 (+)
CBA/J	MGP_CBAJ_G0035790	protein coding gene	ChrX:146641984-146756758 (+)
DBA/2J	MGP_DBA2J_G0035947	protein coding gene	ChrX:136736699-136841482 (+)
FVB/NJ	MGP_FVBNJ_G0035881	protein coding gene	ChrX:135971714-136076089 (+)
LP/J	MGP_LPJ_G0036038	protein coding gene	ChrX:139175421-139274996 (+)
NOD/ShiLtJ	MGP_NODShiLtJ_G0035918	protein coding gene	ChrX:154157638-154282749 (+)
NZO/HlLtJ	MGP_NZOHlLtJ_G0036665	protein coding gene	ChrX:137528364-137638234 (+)
PWK/PhJ	MGP_PWKPhJ_G0034797	protein coding gene	ChrX:115945919-116051098 (+)
SPRET/EiJ	MGP_SPRETEiJ_G0034623	protein coding gene	ChrX:119594748-119713319 (+)
WSB/EiJ	MGP_WSBEiJ_G0035239	protein coding gene	ChrX:135846362-135953636 (+)

Human Ortholog

MID2, midline 2

Vertebrate Orthologs

3

Vertebrate Orthology Source

Alliance of Genome Resources

Human Ortholog

MID2, midline 2
Synonyms

FXY2, MRX101, RNF60, TRIM1, XLID101
Links

HGNC:7096

NCBI Gene ID: 11043

neXtProt AC: NX_Q9UJV3

UniProt: Q9UJV3
Chr Location

Xq22.3; chrX:107825735-107931637 (+) GRCh38

MGI Vertebrate Homology

Mid2 stringent orthology
1 human;1 mouse;1 rat;1 zebrafish
HCOP

vertebrate homology predictions: MID2
Gene Tree

Mid2

Diseases

1 with human MID2 associations

				Human Disease	Mouse Models
				non-syndromic X-linked intellectual disability 101 IDs non-syndromic X-linked intellectual disability 101 DOID:0112048 OMIM:300928

Click on a disease name to see all genes associated with that disease.

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Phenotype Summary

4 phenotypes from 1 allele in 1 genetic background
7 phenotype references

Phenotype Overview

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene.

adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary system	embryo	endocrine/exocrine glands	growth/size/body	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	integument	immune system	limbs/digits/tail	liver/biliary system	mortality/aging	muscle	nervous system	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	taste/olfaction	neoplasm	vision/eye

Click cells to view annotations.

All Mutations and Alleles

5
Endonuclease-mediated

2
Targeted

3
Incidental Mutations

Mutagenetix , APF
Find Mice (IMSR)

7 strains or lines available
Comparison Matrix

Gene Expression + Phenotype

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All GO Annotations

26
GO References

7

Molecular Function

carbohydrate derivative binding	cytoskeletal protein binding	DNA binding	enzyme regulator	hydrolase	ligase	lipid binding	oxidoreductase	RNA binding	signaling receptor activity	signaling receptor binding	transcription	transferase	transporter

Biological Process

carbohydrate derivative metabolism	cell differentiation	cell population proliferation	cellular component organization	DNA-templated transcription	establishment of localization	homeostatic process	immune system process	lipid metabolic process	programmed cell death	protein metabolic process	response to stimulus	signaling	system development

Cellular Component

cell projection	cytoplasmic vesicle	cytoskeleton	cytosol	endoplasmic reticulum	endosome	extracellular region	Golgi apparatus	mitochondrion	membraneless organelle	nucleus	organelle envelope	organelle lumen	plasma membrane	protein-containing complex	synapse	vacuole

Click cells to view annotations.

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Expression Overview

GXD's primary emphasis is on endogenous gene expression during development. Click on grid cells to view annotations.

Blue cells = expressed in wild-type.
Gray triangles = other expression annotations only
(e.g. absence of expression or data from mutants).

early conceptus	embryo ectoderm	embryo endoderm	embryo mesoderm	embryo mesenchyme	extraembryonic component	alimentary system	auditory system	branchial arches	cardiovascular system	connective tissue	endocrine system	exocrine system	hemolymphoid system	integumental system	limbs	liver and biliary system	musculoskeletal system	nervous system	olfactory system	reproductive system	respiratory system	urinary system	visual system

Click cells to view annotations.

Assay Results

944
Tissues

188
cDNA Data

20
Literature Summary

5

Images

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

Other Mouse Links

Allen Institute

GEO

Expression Atlas
Other Vertebrate Links

Xenbase mid2

ZFIN mid2

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All Sequences

47
RefSeq

8
UniProt

5
CCDS

CCDS41151.1

Ensembl

ENSMUSG00000000266 (Evidence)
NCBI Gene

23947

			Length	Strain/Species	Flank
genomic	ENSMUSG00000000266	Ensembl Gene Model \| MGI Sequence Detail	103117	C57BL/6J	± kb
transcript	ENSMUST00000112988	Ensembl \| MGI Sequence Detail	2525	Not Applicable
polypeptide	ENSMUSP00000108612	Ensembl \| MGI Sequence Detail	715	Not Applicable

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UniProt

5 Sequences
Protein Ontology

PR:000010408 midline-2

(term hierarchy)
EC

2.3.2.27
InterPro Domains

IPR001870 B30.2/SPRY domain

IPR043136 B30.2/SPRY domain superfamily

IPR003649 B-box, C-terminal

IPR000315 B-box-type zinc finger

IPR003879 Butyrophylin-like, SPRY domain

IPR013320 Concanavalin A-like lectin/glucanase domain superfamily

IPR017903 COS domain

IPR050617 E3 ubiquitin-protein ligases and FN3/SPRY domain-containing proteins

IPR003961 Fibronectin type III

IPR036116 Fibronectin type III superfamily

IPR013783 Immunoglobulin-like fold

IPR040859 Midline-1, COS domain

IPR047064 Probable E3 ubiquitin-protein ligase MID2, B-box-type 1 zinc finger

IPR047063 Probable E3 ubiquitin-protein ligase MID2, B-box-type 2 zinc finger

IPR033491 Probable E3 ubiquitin-protein ligase MID2, RING finger, HC subclass

IPR003877 SPRY domain

IPR035752 TRIM1, PRY/SPRY domain

IPR047153 TRIM45/56/19

IPR013083 Zinc finger, RING/FYVE/PHD-type

IPR001841 Zinc finger, RING-type

IPR017907 Zinc finger, RING-type, conserved site

IPR027370 Zinc finger, RING-type, eukaryotic
GlyGen

Q9QUS6 2 sites, 2 N-linked glycans (2 sites)

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All nucleic 27

cDNA 20

Primer pair 4

Other 3

Microarray probesets 5

Summaries

All 35

Developmental Gene Expression 5

Gene Ontology 7

Phenotypes 7
Earliest

J:56619 Buchner G, et al., MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development. Hum Mol Genet. 1999 Aug;8(8):1397-407
Latest

J:345621 Adams DJ, et al., Genetic determinants of micronucleus formation in vivo. Nature. 2024 Mar;627(8002):130-136

TSS for Mid2:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr162204	ChrX:139565045-139565075 (+)	-288 bp
Tssr5689	ChrX:139565080-139565091 (+)	-262 bp
Tssr162205	ChrX:139565280-139565303 (+)	-56 bp
Tssr162206	ChrX:139565314-139565350 (+)	-16 bp
Tssr162207	ChrX:139565446-139565478 (+)	114 bp
Tssr162208	ChrX:139565580-139565599 (+)	242 bp
Tssr162209	ChrX:139565620-139565631 (+)	278 bp
Tssr162210	ChrX:139565657-139565691 (+)	326 bp

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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