Eif5b Gene Detail

Summary

• Symbol
  Eif5b
• Name
  eukaryotic translation initiation factor 5B
• Synonyms
  A030003E17Rik, IF2

• Feature Type
  protein coding gene
• IDs
  MGI:2441772
  NCBI Gene: 226982
• Alliance
  gene page
• Transcription Start Sites
  41 TSS

Location &
Maps

• Sequence Map
  Chr1:38037091-38094660 bp, + strand

  From NCBI annotation of GRCm39
  

• View this region in JBrowse
  

• Genome Browsers
  Ensembl | UCSC | NCBI

• Genetic Map
  Chromosome 1, 16.40 cM
• Mapping Data
  2 experiments

Strain
Comparison

• SNPs within 2kb
  1204 from dbSNP Build 142
• Strain Annotations
  18

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_2441772	protein coding gene	Chr1:38037091-38094660 (+)
129S1/SvImJ	MGP_129S1SvImJ_G0015948	protein coding gene	Chr1:36656156-36718050 (+)
A/J	MGP_AJ_G0015932	protein coding gene	Chr1:35645353-35702859 (+)
AKR/J	MGP_AKRJ_G0015887	protein coding gene	Chr1:36136985-36200411 (+)
BALB/cJ	MGP_BALBcJ_G0015886	protein coding gene	Chr1:35303935-35361844 (+)
C3H/HeJ	MGP_C3HHeJ_G0015719	protein coding gene	Chr1:36364918-36429529 (+)
C57BL/6NJ	MGP_C57BL6NJ_G0016339	protein coding gene	Chr1:38245340-38309523 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0014061	protein coding gene	Chr1:32629119-32689233 (+)
CAST/EiJ	MGP_CASTEiJ_G0015300	protein coding gene	Chr1:36221940-36284709 (+)
CBA/J	MGP_CBAJ_G0015691	protein coding gene	Chr1:39305993-39366294 (+)
DBA/2J	MGP_DBA2J_G0015792	protein coding gene	Chr1:34949411-35008231 (+)
FVB/NJ	MGP_FVBNJ_G0015794	protein coding gene	Chr1:34478564-34536983 (+)
LP/J	MGP_LPJ_G0015862	protein coding gene	Chr1:37005299-37064355 (+)
NOD/ShiLtJ	MGP_NODShiLtJ_G0015814	protein coding gene	Chr1:41455519-41513888 (+)
NZO/HlLtJ	MGP_NZOHlLtJ_G0016386	protein coding gene	Chr1:36212394-36270950 (+)
PWK/PhJ	MGP_PWKPhJ_G0015083	protein coding gene	Chr1:34737507-34795791 (+)
SPRET/EiJ	MGP_SPRETEiJ_G0014865	protein coding gene	Chr1:36167695-36218292 (+)
WSB/EiJ	MGP_WSBEiJ_G0015362	protein coding gene	Chr1:36170336-36230122 (+)

Homology

• Human Ortholog
  EIF5B, eukaryotic translation initiation factor 5B

• Vertebrate Orthologs
  3

Vertebrate Orthology Source

Alliance of Genome Resources

• Human Ortholog
  EIF5B, eukaryotic translation initiation factor 5B
  
• Synonyms
  IF2
• Links
  HGNC:30793

  NCBI Gene ID: 9669

  neXtProt AC: NX_O60841

  UniProt: O60841

  
  
• Chr Location
  2q11.2; chr2:99337389-99401326 (+)  GRCh38

---

• MGI Vertebrate Homology
  Eif5b stringent orthology
  1 human;1 mouse;1 rat;1 zebrafish
  
• HCOP
  vertebrate homology predictions: EIF5B
• Gene Tree
  Eif5b
  

Mutations,
Alleles, and
Phenotypes

• Phenotype Summary
  5 phenotypes from 1 allele in 1 genetic background
  1 images
  12 phenotype references
  

• All Mutations and Alleles
  28
• Chemically induced (other)
  1
• Endonuclease-mediated
  2
• Gene trapped
  25
• Genomic Mutations
  1 involving Eif5b
• Incidental Mutations
  Mutagenetix , APF
• Find Mice (IMSR)
  66 strains or lines available

Mice homozygous for a knock-out allele show embryonic growth retardation and lethality before implantation. In vitro outgrowth assays show that embryos fail to hatch from the zona pellucida and die after 3 days in culture, never forming blastocysts.

Gene Ontology
(GO)
Classifications

• All GO Annotations
  21
• GO References
  7

Expression

• Assay Results
  706
• cDNA Data
  21

Tissue x Stage Matrix

• Other Mouse Links
  Allen Institute
  GEO
  Expression Atlas
• Other Vertebrate Links
  GEISHA EIF5B
  Xenbase eif5b
  ZFIN eif5b

Sequences &
Gene Models

• All Sequences
  58
• RefSeq
  4
• UniProt
  5
• CCDS
  CCDS35544.1

• Ensembl
  ENSMUSG00000026083 (Evidence)
• NCBI Gene
  226982

Representative Sequences			Length	Strain/Species
genomic	226982	NCBI Gene Model | MGI Sequence Detail	57570	C57BL/6J
transcript	NM_198303	RefSeq | MGI Sequence Detail	7797	C57BL/6
polypeptide	Q05D44	UniProt | EBI | MGI Sequence Detail	1216	Not Applicable

Protein
Information

• UniProt
  5 Sequences
• Protein Ontology
  PR:000007006 eukaryotic translation initiation factor 5B
  (term hierarchy)
• EC
  3.6.5.3
• InterPro Domains
  IPR029459 Elongation factor Tu-type domain
  IPR027417 P-loop containing nucleoside triphosphate hydrolase
  IPR005225 Small GTP-binding protein domain
  IPR000795 Translational (tr)-type GTP-binding domain
  IPR015760 Translation initiation factor IF- 2
  IPR023115 Translation initiation factor IF- 2, domain 3
  IPR036925 Translation initiation factor IF-2, domain 3 superfamily
  IPR009000 Translation protein, beta-barrel domain superfamily
• GlyGen
  Q05D44 2 sites, 1 O-linked glycan (2 sites)

Molecular
Reagents

• All nucleic 21

  cDNA 21

  
  

  Microarray probesets 5

Other
Accession IDs

MGI:2448517

References

• Summaries
  All 41

  Gene Ontology 7

  Phenotypes 12
• Earliest
  J:80000 The FANTOM Consortium and The RIKEN Genome Exploration Research Group Phase I & II Team, Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs. Nature. 2002;420:563-573
• Latest
  J:345621 Adams DJ, et al., Genetic determinants of micronucleus formation in vivo. Nature. 2024 Mar;627(8002):130-136

TSS for Eif5b:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr6328	Chr1:38037037-38037044 (+)	-50 bp
Tssr6329	Chr1:38037056-38037067 (+)	-29 bp
Tssr49	Chr1:38037068-38037075 (+)	-19 bp
Tssr6330	Chr1:38037086-38037095 (+)	0 bp
Tssr6331	Chr1:38037178-38037195 (+)	96 bp
Tssr6332	Chr1:38037196-38037230 (+)	122 bp
Tssr6333	Chr1:38037233-38037246 (+)	149 bp
Tssr50	Chr1:38037268-38037280 (+)	183 bp
Tssr51	Chr1:38037336-38037354 (+)	254 bp
Tssr52	Chr1:38037375-38037378 (+)	286 bp
Tssr6334	Chr1:38037855-38037858 (+)	766 bp
Tssr6335	Chr1:38056929-38056943 (+)	19,845 bp
Tssr6336	Chr1:38056946-38056958 (+)	19,861 bp
Tssr6337	Chr1:38057127-38057141 (+)	20,043 bp
Tssr6338	Chr1:38057148-38057165 (+)	20,066 bp
Tssr6339	Chr1:38058060-38058104 (+)	20,991 bp
Tssr6340	Chr1:38058108-38058130 (+)	21,028 bp
Tssr6341	Chr1:38058149-38058162 (+)	21,065 bp
Tssr6342	Chr1:38058179-38058190 (+)	21,094 bp
Tssr6343	Chr1:38058196-38058208 (+)	21,111 bp
Tssr6344	Chr1:38058230-38058244 (+)	21,146 bp
Tssr6345	Chr1:38058249-38058273 (+)	21,170 bp
Tssr6346	Chr1:38058312-38058324 (+)	21,227 bp
Tssr6347	Chr1:38058342-38058361 (+)	21,261 bp
Tssr6348	Chr1:38058490-38058501 (+)	21,405 bp
Tssr6349	Chr1:38058529-38058563 (+)	21,455 bp
Tssr6350	Chr1:38060748-38060767 (+)	23,667 bp
Tssr6351	Chr1:38060785-38060803 (+)	23,703 bp
Tssr6352	Chr1:38060811-38060824 (+)	23,727 bp
Tssr6353	Chr1:38060834-38060869 (+)	23,761 bp
Tssr6354	Chr1:38061315-38061335 (+)	24,234 bp
Tssr6355	Chr1:38061354-38061374 (+)	24,273 bp
Tssr6356	Chr1:38071351-38071370 (+)	34,270 bp
Tssr6357	Chr1:38073146-38073157 (+)	36,061 bp
Tssr6358	Chr1:38074774-38074786 (+)	37,689 bp
Tssr6359	Chr1:38074790-38074797 (+)	37,703 bp
Tssr6360	Chr1:38074821-38074830 (+)	37,735 bp
Tssr6361	Chr1:38074838-38074852 (+)	37,754 bp
Tssr6362	Chr1:38074862-38074903 (+)	37,792 bp
Tssr6363	Chr1:38081858-38081872 (+)	44,774 bp
Tssr6364	Chr1:38088324-38088345 (+)	51,244 bp