Tg(Camk2a-Fyn-531)1Nko
Transgene Detail

Summary

• Symbol: Tg(Camk2a-Fyn-531)1Nko
• Name: transgene insertion 1, Nobuhiko Kojima
• MGI ID: MGI:2446470
• Synonyms: fyn+
• Transgene: Tg(Camk2a-Fyn-531)1Nko Location: unknown
• Alliance: Tg(Camk2a-Fyn-531)1Nko page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Constitutively active, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Camk2a-Fyn-531)1Nko expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Fyn (MGI:95602)	Expresses a Fyn isoform B cDNA carrying a TAT to TTT nucleotide substitution at codon 531

• Tg(Camk2a-Fyn-531)1Nko expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Camk2a (MGI:88256)

• Mutation details: The transgene consists of a Camk2a promoter fused to a Fyn isoform B cDNA carrying a TAT to TTT nucleotide substitution at codon 531. Camk2a drives expression in forebrain neurons late in development. This mutation renders Fyn constitutively activated. Transgene expression in the brain was confirmed by Northern blot, RNase protection assay, RT-PCR, and in situ hybridization. (J:59490)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Six transgenic lines (M3, M27, M47, M58, M78, and M84) carrying the mutant Fyn gene were created. Mice from lines M3, M47, and M84 showed higher mortality (died at 8, 5, and 15 weeks, repectively), and bred poorly and were excluded from biochemical analysis. Line M58 displayed the lowest amount of transgenic protein.

References

• Original: J:59490 Kojima N, et al., Higher seizure susceptibility and enhanced tyrosine phosphorylation of N-methyl-D-aspartate receptor subunit 2B in fyn transgenic mice. Learn Mem. 1998 Nov-Dec;5(6):429-45
• All: 3 reference(s)