Tg(RHO-SCA7)R7EJman
Transgene Detail

Summary

• Symbol: Tg(RHO-SCA7)R7EJman
• Name: transgene insertion R7E, Jean-Louis Mandel
• MGI ID: MGI:2447845
• Synonyms: R7E
• Transgene: Tg(RHO-SCA7)R7EJman Location: unknown
• Alliance: Tg(RHO-SCA7)R7EJman page

Aberrant retinal rod chromatin remodeling and segment loss in Tg(RHO-SCA7)R7EJman/0 mice compared to normal retinas in Tg(RHO-SCA7)R7NJman/0 mice

Show the 2 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: C57BL/6 x SJL

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(RHO-SCA7)R7EJman expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ATXN7 (6314)

  Atxn7

  (MGI:2179277)

• Mutation details: The transgene consists of a human rhodopsin promoter (containing the RER enhancer element), the full-length mutant human Sca7 gene carrying a 90 glutamine repeat expansion, and an SV40 intron and polyadenylation sequence. Transgene expression was observed in rod cells by immunofluorescence, and in retinal extracts by Western blot. (J:65411)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Transgenic lines A, B, C, D, and F were constructed. Line A carries 10-50 copies of the integrated transgene and line F carries 1-5 copies. Lines A and F express the highest amount of transgene product and were used for analysis.

References

• Original: J:65411 Yvert G, et al., Expanded polyglutamines induce neurodegeneration and trans-neuronal alterations in cerebellum and retina of SCA7 transgenic mice. Hum Mol Genet. 2000 Oct 12;9(17):2491-506
• All: 8 reference(s)