Tg(PLADA)4118Rkmb
Transgene Detail

Summary

• Symbol: Tg(PLADA)4118Rkmb
• Name: transgene insertion 4118, Michael R Blackburn
• MGI ID: MGI:2683027
• Transgene: Tg(PLADA)4118Rkmb Location: unknown
• Alliance: Tg(PLADA)4118Rkmb page

Lung, bone and kidney abnormalities are observed in Ada^tm1Mw/Ada^tm1Mw Tg(PLADA)4118Rkmb/0 mice

Show the 2 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(PLADA)4118Rkmb expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ada (MGI:87916)

• Mutation details: A construct was made that contained the endogenous polyadenylation sequence, intron 11, and ~2kb of of the 3' flanking region of the gene. The endogenous promoter was replaced with a promoter containing a 36-bp deletion in the 5'-untranslated region and 770-bp trophoblast regulatory element. This resulted in a construct that allowed expression in the placenta prenatally only, while the 36-bp deletion in the promoter allowed distinction of this transcript from that of native transcript. Enzymatic activity of the protein product was not detected in any of the tissues tested at postnatal day 17 in transgenic mice. (J:73418)

Disease models

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:73418 Blackburn MR, et al., Adenosine deaminase-deficient mice generated using a two-stage genetic engineering strategy exhibit a combined immunodeficiency. J Biol Chem. 1998 Feb 27;273(9):5093-100
• All: 10 reference(s)